Polyclonal Antibody to Neurofilament, Light Polypeptide (NEFL) | MyBioSource MBS2006101 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Neurofilament, Light Polypeptide (NEFL)

catalog :

MBS2006101

quantity :

0.01 mg

price :

110 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Figure. Western Blot; Sample: Recombinant NEFL, Human.

image 2 :

Western Blot; Sample: Rat Cerebrum lysate;. Primary Ab: 1ug/ml Rabbit Anti-Human NEFL Antibody. Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody. (Catalog: MBS2086047)

image 3 :

Western Blot; Sample: Mouse Cerebrum lysate;. Primary Ab: 1ug/ml Rabbit Anti-Human NEFL Antibody. Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody. (Catalog: MBS2086047)

product information

catalog number :

MBS2006101

products type :

Antibody

products full name :

Polyclonal Antibody to Neurofilament, Light Polypeptide (NEFL)

products short name :

[Neurofilament, Light Polypeptide (NEFL)]

other names :

[neurofilament light polypeptide; Neurofilament light polypeptide; neurofilament light polypeptide; neurofilament light; 68 kDa neurofilament protein; Neurofilament triplet L protein]

products gene name :

[NEFL]

other gene names :

[NEFL; NEFL; NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110; NF68; NFL; NF-L]

clonality :

Polyclonal

host :

Rabbit

purity :

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

form :

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

concentration :

0.69 mg/ml

storage stability :

Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP)

app notes :

Western blotting: 0.5-2 ug/mL. Immunohistochemistry: 5-20 ug/mL . Immunocytochemistry: 5-20 ug/mL. Optimal working dilutions must be determined by end user

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Western Blot (WB)

other info1 :

Organism Species: Homo sapiens (Human). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant NEFL (Lys281~Glu396) expressed in E.coli

ncbi gi num :

105990539

ncbi acc num :

NP_006149.2

ncbi gb acc num :

NM_006158.4

uniprot acc num :

P07196

ncbi pathways :

Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); CREB Phosphorylation Through The Activation Of CaMKII Pathway (161040); CREB Phosphorylation Through The Activation Of Ras Pathway (161036); Neuronal System Pathway (106513); Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway (106534); Post NMDA Receptor Activation Events Pathway (161035); Ras Activation Uopn Ca2+ Infux Through NMDA Receptor Pathway (161037)

ncbi summary :

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

uniprot summary :

NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F). Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 8p21.2. Cellular Component: axon; axon cytoplasm; cytoplasm; cytosol; growth cone; myelin sheath; neurofilament; TSC1-TSC2 complex. Molecular Function: identical protein binding; phospholipase binding; protein binding; protein binding, bridging; protein C-terminus binding; protein domain specific binding; protein heterodimerization activity; Ras guanyl-nucleotide exchange factor activity; structural constituent of cytoskeleton. Biological Process: anterograde axon cargo transport; axon regeneration in the peripheral nervous system; axonal transport of mitochondrion; cerebral cortex development; hippocampus development; intermediate filament organization; intermediate filament polymerization and/or depolymerization; locomotion; MAPK cascade; microtubule cytoskeleton organization; negative regulation of neuron apoptosis; neurofilament bundle assembly; neuromuscular process controlling balance; neuron projection morphogenesis; positive regulation of axonogenesis; protein polymerization; regulation of axon diameter; regulation of molecular function; response to acrylamide; response to corticosterone stimulus; response to peptide hormone; response to sodium arsenite; response to toxin; retrograde axon cargo transport; spinal cord development. Disease: Charcot-marie-tooth Disease, Axonal, Type 2e; Charcot-marie-tooth Disease, Demyelinating, Type 1f

size1 :

0.01 mg

price1 :

110 USD

size2 :

0.02 mg

price2 :

130

size3 :

0.05 mg

price3 :

175

size4 :

0.1 mg

price4 :

225

size5 :

0.2 mg

price5 :

340

size6 :

1 mg

price6 :

800

more info or order :

MyBioSource product webpage