Polyclonal Antibody to Pregnane X Receptor (PXR) | MyBioSource MBS2006061 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Pregnane X Receptor (PXR)

catalog :

MBS2006061

quantity :

0.01 mg

price :

110 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Recombinant protein.

image 2 :

DABstainingonIHC-P.Samples:MouseTissue)

product information

catalog number :

MBS2006061

products type :

Antibody

products full name :

Polyclonal Antibody to Pregnane X Receptor (PXR)

products short name :

[Pregnane X Receptor (PXR)]

other names :

[nuclear receptor subfamily 1 group I member 2 isoform 2; Nuclear receptor subfamily 1 group I member 2; nuclear receptor subfamily 1 group I member 2; nuclear receptor subfamily 1, group I, member 2; Orphan nuclear receptor PXR; Pregnane X receptor]

products gene name :

[PXR]

other gene names :

[Nr1i2; Nr1i2; PXR; SXR; PXR1; mPXR; PXR.1; PXR.2; Pxr]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mus musculus (Mouse)

sequence length :

411

sequence :

Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- YMFKGV INFAKVISYF RDLPIEDQIS LLKGATFEMC ILRFNTMFDT ETGTWECGRL AYCFEDPNGG FQKLLLDPLM KFHCMLKKLQ LHKEEYVLMQ AISLFSPDRP GVVQRSVVDQ LQERFALTLK AYIECSRPYP AHRFLFLKIM AVLTELRSIN AQQTQQLLRI QDSHPFATPL MQELFSSTDG

specificity :

The antibody is a rabbit polyclonal antibody raised against PXR. It has been selected for its ability to recognize PXR in immunohistochemical staining andwestern blotting.

purity :

Antigen-specific affinity chromatography

form :

Supplied as solution form in 0.01M PBS, pH1.4, containing 0.05% Proclin-300, 50% glycerol.

concentration :

200ug/ml

storage stability :

Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months. Stability Test:. The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 3rC for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, Immunoprecipitation (IP), Western Blot (WB)

app notes :

Western blotting: 0.5-2ug/mL. Immunohistochemistry: 5-20ug/mL. Immunocytochemistry: 5-20ug/mL. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant PXR (Tyr205-Gly390) expressed in E.coli.

other info2 :

Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2073698

ncbi gi num :

148536879

ncbi acc num :

NP_001091874.1

ncbi gb acc num :

NM_001098404.1

uniprot acc num :

O54915

ncbi mol weight :

44,958 Da

ncbi pathways :

Gene Expression Pathway (574654); Generic Transcription Pathway (574655); Nuclear Receptor Transcription Pathway (574657); Nuclear Receptors Pathway (198318); Nuclear Receptors In Lipid Metabolism And Toxicity Pathway (198356)

uniprot summary :

PXR: Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes. Heterodimer with RXR. Interacts with NCOA1. Expressed in liver, colon and small intestine. Belongs to the nuclear hormone receptor family. NR1 subfamily. 7 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Nuclear receptor. Chromosomal Location of Human Ortholog: 16 16 B3. Cellular Component: intermediate filament cytoskeleton; nuclear body; nucleoplasm; nucleus. Molecular Function: DNA binding; DNA binding transcription factor activity; drug binding; ligand-dependent nuclear receptor activity; metal ion binding; protein binding; sequence-specific DNA binding; steroid hormone receptor activity; zinc ion binding. Biological Process: drug export; exogenous drug catabolic process; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of transcription, DNA-templated; steroid hormone mediated signaling; transcription, DNA-dependent; xenobiotic metabolic process; xenobiotic transport

size1 :

0.01 mg

price1 :

110 USD

size2 :

0.02 mg

price2 :

130

size3 :

0.05 mg

price3 :

185

size4 :

0.1 mg

price4 :

230

size5 :

0.2 mg

price5 :

345

size6 :

1 mg

price6 :

825

more info or order :

MyBioSource product webpage