Polyclonal Antibody to Lumican (LUM) | MyBioSource MBS2005925 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Lumican (LUM)

catalog :

MBS2005925

quantity :

0.01 mg

price :

130 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2005925

products type :

Antibody

products full name :

Polyclonal Antibody to Lumican (LUM)

products short name :

Lumican (LUM)

other names :

lumican; Lumican; lumican; KSPG lumican; lumican proteoglycan; keratan sulfate proteoglycan lumican; lumican; Keratan sulfate proteoglycan lumican

products gene name :

LUM

other gene names :

LUM; LUM; LDC; SLRR2D; LDC; SLRR2D; KSPG lumican

uniprot entry name :

LUM_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

338

sequence :

The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF-QY YDYDIPLFMY GQISPNCAPE CNCPHSYPTA MYCDDLKLKS VPMVPPGIKY LYLRNNQIDH IDEKAFENVT DLQWLlLDHN LLENSKIKGK VFSKLKQLKK LHINYNNLTE SVGPLPKSLQ DLQLTNNKIS KLGSFDGLVN LTFIYLQHNQ LKEDAVSASL KGLKSLEYLD LSFNQMSKLP AGLPTSLLTL YLDNNKISNI PDEYFKRFTG LQYLRLSHNE LADSGVPGNS FNISSLLELD LSYNKLKSIP TVNENLENYY LEVNELEKFD VKSFCKILGP LSYSKIKHLR LDGNPLTQSS LPPDMYECLR V,Il,NEITVN

specificity :

The antibody is a rabbit polyclonal antibody raised against LUM. It has been selected for its ability to recognize LUM in immunohistochemical staining andwestern blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml. UOM: 100ug

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200

other info1 :

Immunogen: Recombinant LUM (Gln19~Asn338) expressed in E.coli.

other info2 :

Quality Control: Content: The quality control contains recombinant CRP (Phe17~Pro224) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

ncbi gi num :

4505047

ncbi acc num :

NP_002336.1

ncbi gb acc num :

NM_002345.3

uniprot acc num :

P51884

ncbi mol weight :

38,429 Da

ncbi pathways :

Disease Pathway (530764); ECM Proteoglycans Pathway (833812); Extracellular Matrix Organization Pathway (576262); Glycosaminoglycan Metabolism Pathway (645297); Integrin Cell Surface Interactions Pathway (106110); Keratan Sulfate Biosynthesis Pathway (645302); Keratan Sulfate Degradation Pathway (645303); Keratan Sulfate/keratin Metabolism Pathway (645301); MPS I - Hurler Syndrome Pathway (685537); MPS II - Hunter Syndrome Pathway (685538)

ncbi summary :

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]

uniprot summary :

LUM: a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 12q21.33. Cellular Component: extracellular matrix; lysosomal lumen; extracellular space; proteinaceous extracellular matrix; fibrillar collagen; Golgi lumen; extracellular region. Molecular Function: collagen binding; protein binding; extracellular matrix structural constituent. Biological Process: keratan sulfate metabolic process; extracellular matrix organization and biogenesis; collagen fibril organization; glycosaminoglycan metabolic process; visual perception; cartilage development; keratan sulfate biosynthetic process; carbohydrate metabolic process; positive regulation of transcription from RNA polymerase II promoter; pathogenesis; keratan sulfate catabolic process; response to organic cyclic substance

size1 :

0.01 mg

price1 :

130 USD

size2 :

0.05 mg

price2 :

225

size3 :

0.1 mg

price3 :

295

more info or order :

MyBioSource product webpage