catalog number :
MBS2005813
products full name :
Polyclonal Antibody to Gastrokine 2 (GKN2)
products short name :
Gastrokine 2 (GKN2)
other names :
gastrokine-2; Gastrokine-2; gastrokine-2; blottin; BRICHOS domain containing 1B; trefoil factor interactions(z) 1; down regulated in gastric cancer GDDR; down-regulated in gastric cancer GDDR; gastrokine 2; Blottin; Down-regulated in gastric cancer; Trefoil factor interactions(z) 1
products gene name :
GKN2
other gene names :
GKN2; GKN2; GDDR; TFIZ1; PRO813; BRICD1B; VLTI465; BLOT; GDDR; TFIZ1
uniprot entry name :
GKN2_HUMAN
sequence :
The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF-YEVFNIISPS NNGGNVQETV TIDNEKNTAI INIHAGSCSS TTIFDYKHGY IASRVLSRRA CFILKMDHQN IPPLNNLQWY IYEKQALDNM FSSKYTWVKY NPLESLIKDV DWFLLGSPIE KLCKHIPLYK GEVVENTHNV GAGGCAKAGL LGILGISICA DIHV
specificity :
The antibody is a rabbit polyclonal antibody raised against GKN2. It has been selected for its ability to recognize GKN2 in immunohistochemical staining andwestern blotting.
purity :
Affinity Chromatography
form :
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
storage stability :
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
tested application :
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
app notes :
Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200
other info1 :
Immunogen: Recombinant GKN2 (Tyr21~Val184) expressed in E.coli.
other info2 :
Quality Control: Content: The quality control contains recombinant CRP (Phe17~Pro224) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
ncbi acc num :
NP_872342.2
ncbi gb acc num :
NM_182536.2
ncbi mol weight :
20,487 Da
uniprot summary :
GKN2: Interacts with TFF2. Heterodimer with TFF1; disulfide linked. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 2p13.3. Cellular Component: extracellular region