Polyclonal Antibody to Transglutaminase 2, Tissue (TGM2) | MyBioSource MBS2005631 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Transglutaminase 2, Tissue (TGM2)

catalog :

MBS2005631

quantity :

0.01 mg

price :

105 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Mouse Placenta lysate; Primary Ab: 1ug/ml Rabbit Anti-Human TGM2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

image 2 :

Western Blot: Sample: Recombinant protein.

image 3 :

DAB staining on fromalin fixed paraffin-embedded Liver tissue)

product information

catalog number :

MBS2005631

products type :

Antibody

products full name :

Polyclonal Antibody to Transglutaminase 2, Tissue (TGM2)

products short name :

[Transglutaminase 2, Tissue (TGM2)]

other names :

[protein-glutamine gamma-glutamyltransferase 2 isoform a; Protein-glutamine gamma-glutamyltransferase 2; protein-glutamine gamma-glutamyltransferase 2; transglutaminase 2; Tissue transglutaminase; Transglutaminase C; TG(C); TGC; TGase C; Transglutaminase H; TGase H; Transglutaminase-2; TGase-2]

products gene name :

[TGM2]

other gene names :

[TGM2; TGM2; TGC; TG(C); TG(C); TGC; TGase C; TGase H; TGase-2]

clonality :

Polyclonal

host :

Rabbit

sequence length :

687

purity :

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

concentration :

1 mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Western Blot(WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP).

app notes :

Western blotting: 0.5-2 ug/mL;1:500-2000. Immunohistochemistry: 5-20 ug/mL;1:50-200. Immunocytochemistry: 5-20 ug/mL;1:50-200. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Knockout Validation

image4 description :

Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: TGM2 knockout Hela cell lysate; Predicted MW: 77kd Observed MW: 77kd Primary Ab: 1ug/ml Rabbit Anti-Human TGM2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)

other info1 :

Organism Species: Homo sapiens (Human). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant TGM2 (Met1~Ala687 (Accession # P21980)) expressed in E.coli

other info2 :

Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2059642

products categories :

Knock-Out Validation Antibody

ncbi gi num :

1020158911

ncbi acc num :

NP_001310245.1

ncbi gb acc num :

NM_001323316.1

uniprot acc num :

P21980

ncbi pathways :

Huntington's Disease Pathway (83100); Huntington's Disease Pathway (512); Thromboxane A2 Receptor Signaling Pathway (138060)

ncbi summary :

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

TGM2: an enzyme of the transglutaminase family that catalyzes the crosslinking of proteins and the conjugation of polyamines to proteins. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Is the autoantigen in coeliac disease and plays a role in apoptosis, cellular differentiation and matrix stabilisation. Three alternatively spliced isoforms have been described. Protein type: EC 2.3.2.13; Transferase. Chromosomal Location of Human Ortholog: 20q11.23. Cellular Component: cytosol; endoplasmic reticulum; extracellular exosome; extracellular matrix; focal adhesion; intrinsic component of plasma membrane; mitochondrion. Molecular Function: GTP binding; metal ion binding; protein binding; protein domain specific binding; protein-glutamine gamma-glutamyltransferase activity. Biological Process: apoptotic cell clearance; blood vessel remodeling; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; negative regulation of endoplasmic reticulum calcium ion concentration; positive regulation of apoptosis; positive regulation of cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of inflammatory response; positive regulation of mitochondrial calcium ion concentration; positive regulation of smooth muscle cell proliferation; protein homooligomerization

size1 :

0.01 mg

price1 :

105 USD

size2 :

0.02 mg

price2 :

125

size3 :

0.05 mg

price3 :

170

size4 :

0.1 mg

price4 :

220

size5 :

0.2 mg

price5 :

320

size6 :

1 mg

price6 :

760

more info or order :

MyBioSource product webpage