antibody

Polyclonal Antibody to A Disintegrin And Metalloprotease 5 (ADAM5)

MBS2005629

0.05 mg

340 USD

polyclonal

rabbit

nonconjugated

human, rat

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

Antibody

Polyclonal Antibody to A Disintegrin And Metalloprotease 5 (ADAM5)

A Disintegrin And Metalloprotease 5 (ADAM5)

A disintegrin and metalloproteinase with thrombospondin motifs 5 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 5; A disintegrin and metalloproteinase with thrombospondin motifs 5; aggrecanase-2; a disintegrin and metalloproteinase with thrombospondin motifs 11; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2); ADAM metallopeptidase with thrombospondin type 1 motif, 5; A disintegrin and metalloproteinase with thrombospondin motifs 11; ADAM-TS 11; ADAMTS-11; ADMP-2; Aggrecanase-2

ADAM5

ADAMTS5; ADAMTS5; ADMP-2; ADAM-TS5; ADAMTS-5; ADAMTS11; ADAM-TS 5; ADAMTS-11; ADAM-TS 11; ADAMTS11; ADMP2; ADAM-TS 5; ADAM-TS5; ADAMTS-5; ADAM-TS 11; ADAMTS-11

ATS5_HUMAN

Polyclonal

IgG

Rabbit

Rat

930

The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF-CDFEEYCN GNDAYCVPDT FARNGQYCDS GQAFCYSGLC MTSNNQCMNL LGKYVRGASF ACYEEFNSRN DRFGNCIRKF CSFENSLCGK LVCTWPFKRL LMKDNMSAAY GQIRDDLCIS LYKGGRPLKT TLSTYSDMSE RDETFVKDGT ICGPDMFCLE TQCKETRFLV DFQQCNTSRD CNDHGVCNNF NHCHCDKGYN PPYCESVKGQ FGSIDDGHKY YIDEGKSAKQ QNRGIHPKQ

The antibody is a rabbit polyclonal antibody raised against ADAM5. It has been selected for its ability to recognize ADAM5 in immunohistochemical staining andwestern blotting.

Affinity Chromatography

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

200ug/ml

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200

Immunogen: Recombinant ADAM5 (Cys413~Gln649) expressed in E.coli.

Quality Control: Content: The quality control contains recombinant Thyroglobulin (Ser2083~Ala2333) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

195539372

NP_008969.2

NM_007038.3

Q9UNA0

101,718 Da

Degradation Of The Extracellular Matrix Pathway 576263!!Degradation Of The Extracellular Matrix Pathway 934113!!Endochondral Ossification Pathway 198812!!Extracellular Matrix Organization Pathway 576262!!Extracellular Matrix Organization Pathway 934100

Function: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period. Catalytic activity: Cleaves aggrecan at the 392-Glu- -Ala-393 site. Cofactor: Binds 1 zinc ion per subunit. Ref.6. Subcellular location: Secreted extracellular space extracellular matrix . By similarity. Tissue specificity: Expressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient. Domain: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. Post-translational modification: The precursor is cleaved by a furin endopeptidase . By similarity.C- and O-glycosylated. O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation can mediate the efficient secretion of ADAMTS family members. Can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion . By similarity. Ref.5 Ref.6Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion . By similarity. Ref.5 Ref.6. Sequence similarities: Contains 1 disintegrin domain.Contains 1 peptidase M12B domain.Contains 2 TSP type-1 domains.

0.05 mg

340 USD