Polyclonal Antibody to Clusterin (CLU) | MyBioSource MBS2005329 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Clusterin (CLU)

catalog :

MBS2005329

quantity :

0.01 mg

price :

145 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2005329

products type :

Antibody

products full name :

Polyclonal Antibody to Clusterin (CLU)

products short name :

Clusterin (CLU)

other names :

clusterin preproprotein; Clusterin; clusterin; apolipoprotein J; ku70-binding protein 1; sulfated glycoprotein 2; aging-associated protein 4; complement lysis inhibitor; complement cytolysis inhibitor; complement-associated protein SP-40,40; testosterone-repressed prostate message 2; clusterin; Aging-associated gene 4 protein; Apolipoprotein J; Apo-J; Complement cytolysis inhibitor; CLI; Complement-associated protein SP-40,40; Ku70-binding protein 1; NA1/NA2; Testosterone-repressed prostate message 2; TRPM-2Cleaved into the following 2 chains:Clusterin beta chain; Alternative name(s):; ApoJalpha; Complement cytolysis inhibitor a chainClusterin alpha chain; Alternative name(s):; ApoJbeta; Complement cytolysis inhibitor b chain

products gene name :

CLU

other gene names :

CLU; CLU; CLI; AAG4; APOJ; CLU1; CLU2; KUB1; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2; APOJ; CLI; KUB1; Apo-J; CLI; TRPM-2

uniprot entry name :

CLUS_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Porcine

sequence length :

449

sequence :

The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSGS-IM PFPLFTDLNY HDMFQPFFDM IHQAQQAMDA HLHRIPYHFP EAGVPENSND RAVCKEIRHN STGCLRMKDQ CEKCREILSV DCSASNSSQM QLRQELYTSL QMAEKFSKLY DQLLQSYQQK MLNTSSLLKQ LNEQFSWVSQ LANLTQNDDR YYLQVTTVNS HGSDPSVPSG LTKVVVKLFD SYPITLIIPQ EVSDPKFMET VAEEALQQYR QRSREE

specificity :

The antibody is a rabbit polyclonal antibody raised against CLU. It has been selected for its ability to recognize CLU in immunohistochemical staining andwestern blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:50-400. Immunocytochemistry in formalin fixed cells: 1:50-500. Immunohistochemistry in formalin fixed frozen section: 1:50-500. Immunohistochemistry in paraffin section: 1:10-100. Enzyme-linked Immunosorbent Assay: 1:100-200. Optimal working dilutions must be determined by end user.

other info1 :

Immunogen: CLU, Porcine. Immunogen information: Recombinant CLU (Ile229~Glu446) with N-terminal His-Tag expressed in E.coli. Accession No.: RPB180Po01. UOM: 100ug

other info2 :

Quality Control: Content: The quality control contains recombinant MUC5B (Lys5366~Val5444) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

ncbi gi num :

355594753

ncbi acc num :

NP_001822.3

ncbi gb acc num :

NM_001831.3

uniprot acc num :

P10909

ncbi mol weight :

52,495 Da

ncbi pathways :

Hemostasis Pathway (106028); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048); Validated Targets Of C-MYC Transcriptional Repression Pathway (169353)

ncbi summary :

The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011]

uniprot summary :

CLU: Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress- induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. Belongs to the clusterin family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Apoptosis; Secreted; Mitochondrial. Chromosomal Location of Human Ortholog: 8p21-p12. Cellular Component: Golgi apparatus; extracellular matrix; extracellular space; mitochondrion; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; mitochondrial membrane; extracellular region; cytosol; nucleus. Molecular Function: protein binding; ubiquitin protein ligase binding; chaperone binding; ATPase activity; misfolded protein binding. Biological Process: platelet activation; release of cytochrome c from mitochondria; response to misfolded protein; positive regulation of nitric oxide biosynthetic process; protein stabilization; positive regulation of apoptosis; response to virus; cell morphogenesis; microglial cell activation; positive regulation of tumor necrosis factor production; activation of NF-kappaB transcription factor; complement activation; negative regulation of protein homooligomerization; reverse cholesterol transport; platelet degranulation; myelin maintenance in the central nervous system; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein import; innate immune response; lipid metabolic process; blood coagulation; chaperone-mediated protein complex assembly; complement activation, classical pathway

size1 :

0.01 mg

price1 :

145 USD

size2 :

0.05 mg

price2 :

260

size3 :

0.1 mg

price3 :

360

more info or order :

MyBioSource product webpage