Polyclonal Antibody to Prominin 1 (PROM1) | MyBioSource MBS2005318 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Prominin 1 (PROM1)

catalog :

MBS2005318

quantity :

0.01 mg

price :

105 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Recombinant PROM1, Human.

image 2 :

DABstainingonIHC-P.Samples:HumanTissue)

product information

catalog number :

MBS2005318

products type :

Antibody

products full name :

Polyclonal Antibody to Prominin 1 (PROM1)

products short name :

[Prominin 1 (PROM1)]

other names :

[prominin-1 isoform 2; Prominin-1; prominin-1; prominin 1; Antigen AC133; Prominin-like protein 1; CD_antigen: CD133]

products gene name :

[PROM1]

other gene names :

[PROM1; PROM1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061; PROML1]

clonality :

Polyclonal

host :

Rabbit

purity :

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

form :

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

concentration :

1 mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

WB; IHC; ICC; IP.

app notes :

Western blotting: 0.5-2 Ug/mL;1:500-2000. Immunohistochemistry: 5-20 Ug/mL;1:50-200. Immunocytochemistry: 5-20 Ug/mL;1:50-200. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Organism Species: Homo sapiens (Human). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant PROM1 (Ile171~Asp431) expressed in E.coli

other info2 :

Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2056128

ncbi gi num :

224994189

ncbi acc num :

NP_001139319.1

ncbi gb acc num :

NM_001145847.1

uniprot acc num :

O43490

ncbi pathways :

Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

ncbi summary :

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

uniprot summary :

CD133: Binds cholesterol in cholesterol-containing plasma membrane microdomains. Proposed to play a role in apical plasma membrane organization of epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. Interacts with CDHR1 and with actin filaments. Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells. Expressed in adult retina by rod and cone photoreceptor cells. Belongs to the prominin family. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Cell surface; Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 4p15.32. Cellular Component: apical plasma membrane; cell surface; endoplasmic reticulum; endoplasmic reticulum-Golgi intermediate compartment; extracellular space; integral component of plasma membrane; microvillus membrane; photoreceptor outer segment; plasma membrane; vesicle. Molecular Function: actinin binding; cadherin binding; protein binding. Biological Process: photoreceptor cell maintenance; retina morphogenesis in camera-type eye. Disease: Cone-rod Dystrophy 12; Macular Dystrophy, Retinal, 2; Retinitis Pigmentosa 41; Stargardt Disease 4

size1 :

0.01 mg

price1 :

105 USD

size2 :

0.02 mg

price2 :

125

size3 :

0.05 mg

price3 :

170

size4 :

0.1 mg

price4 :

220

size5 :

0.2 mg

price5 :

320

size6 :

1 mg

price6 :

760

more info or order :

MyBioSource product webpage