Polyclonal Antibody to Deiodinase, Iodothyronine, Type III (DIO3) | MyBioSource MBS2005140 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Deiodinase, Iodothyronine, Type III (DIO3)

catalog :

MBS2005140

quantity :

0.01 mg

price :

140 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2005140

products type :

Antibody

products full name :

Polyclonal Antibody to Deiodinase, Iodothyronine, Type III (DIO3)

products short name :

Deiodinase, Iodothyronine, Type III (DIO3)

other names :

Deiodinase, iodothyronine, type III; Type III iodothyronine deiodinase; type III iodothyronine deiodinase; type 3 DI; type-III 5' deiodinase; type-III 5'-deiodinase; type 3 iodothyronine selenodeiodinase; iodothyronine deiodinase, placental type; thyroxine deiodinase type III (selenoprotein); deiodinase, iodothyronine, type III; 5DIII; DIOIII; Type 3 DI; Type-III 5'-deiodinase

products gene name :

DIO3

other gene names :

DIO3; DIO3; D3; 5DIII; TXDI3; DIOIII; ITDI3; TXDI3

uniprot entry name :

IOD3_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Rat

sequence length :

278

sequence :

The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE ALKDAQTGST SGSGHHHHHH SAGLVPRGST AIGMKETAAA KFERQHMDSP DLGTLEVLFQ GPLGSEF- RLCAQTASCL VLFPRFLGTA FMLWLLDFLC IRKHFLRRRH PDHPEPEVEL NSEGEEMPPD DPPICVSDDN RLCTLASLKA VWHGQKLDFF KQAHEGGPAPã€€ NSEVVRPDGF QSQRILDYAQ GTRPLVLNFG SCTUPPFMAR MSAFQRLVTK YQRDVDFLII YIEEAHPSDG WVTTDSPYVI PQHRSLEDRV SAARVLQQGA PGCALVLDTM ANSSSSAYGA YFERLYVIQS GTIMYQGGRG PDGYQVSELR TWLERYDEQL HGTRPRR

specificity :

The antibody is a rabbit polyclonal antibody raised against DIO3. It has been selected for its ability to recognize DIO3 in immunohistochemical staining andwestern blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200

other info1 :

Immunogen: Recombinant DIO3 (Arg11~Arg277) expressed in E.coli.

other info2 :

Quality Control: Content: The quality control contains recombinant Thyroglobulin (Ser2083~Ala2333) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

ncbi gi num :

56103188

ncbi acc num :

AAH17717.2

uniprot acc num :

P55073

ncbi mol weight :

31,451 Da

ncbi pathways :

Amine-derived Hormones Pathway (160983); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Regulation Of Thyroid Hormone Activity Pathway (160986); Selenium Metabolism And Selenoproteins Pathway (198846); Selenium Pathway (198825); Thyroxine Biosynthesis Pathway (160985); Thyroid Hormone Metabolism I (via Deiodination) Pathway (545360); Thyroid Hormone Metabolism I (via Deiodination) Pathway (139512); Thyronamine And Iodothyronamine Metabolism Pathway (545636)

ncbi summary :

The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. This protein contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]

uniprot summary :

DIO3: Responsible for the deiodination of T4 (3,5,3 ,5 - tetraiodothyronine) into RT3 (3,3 ,5 -triiodothyronine) and of T3 (3,5,3 -triiodothyronine) into T2 (3,3 -diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development. Belongs to the iodothyronine deiodinase family. Protein type: Oxidoreductase; Membrane protein, integral; EC 1.97.1.11. Chromosomal Location of Human Ortholog: 14q32. Cellular Component: plasma membrane; integral to membrane; endosome membrane. Molecular Function: thyroxine 5 -deiodinase activity; thyroxine 5-deiodinase activity. Biological Process: thyroid hormone catabolic process; thyroid hormone generation; positive regulation of multicellular organism growth; hormone biosynthetic process

size1 :

0.01 mg

price1 :

140 USD

size2 :

0.05 mg

price2 :

250

size3 :

0.1 mg

price3 :

345

more info or order :

MyBioSource product webpage