Biotin-Linked Antibody to Osteonectin (ON) | MyBioSource MBS2005086 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Biotin-Linked Antibody to Osteonectin (ON)

catalog :

MBS2005086

quantity :

0.1 mg

price :

355 USD

clonality :

polyclonal

host :

rabbit

conjugate :

biotin

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2005086

products type :

Antibody

products full name :

Biotin-Linked Antibody to Osteonectin (ON)

products short name :

Osteonectin (ON)

other names :

osteonectin; SPARC; SPARC; BM-40; osteonectin; cysteine-rich protein; basement-membrane protein 40; secreted protein acidic and rich in cysteine; secreted protein, acidic, cysteine-rich (osteonectin); Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine

products gene name :

other gene names :

SPARC; SPARC; ON; ON; BM-40; ON

uniprot entry name :

SPRC_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence :

Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- APQ QEALPDETEV VEETVAEVTE VSVGANPVQV EVGEFDDGAE ETEEEVVAEN PCQNHHCKHG KVCELDENNT PMCVCQDPTS CPAPIGEFEK VCSNDNKTFD SSCHFFATKC TLEGTKKGHK LHLDYIGPCK YIPPCLDSEL TEFPLRMRDW LKNVLVTLYE RDEDNNLLTE KQKLRVKKIH ENEKRLEAGD HPVELLARDF EKNYNMYIFP VHWQFGQLDQ HPIDGYLSHT ELAPLRAPLI PMEHCTTRFF ETCDLDNDKY IALDEWAGCF GIKQKDIDKD LVI

specificity :

The antibody is a rabbit polyclonal antibody raised against ON conjugated to biotin. It has beenselected for its ability to recognize ON in immunohistochemical staining andwestern blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC), (IHC-f) & (IHC-P), Immunofluorescence (IF), Western Blot (WB)

app notes :

Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200

other info1 :

Immunogen: Osteonectin, Human

other info2 :

Conjugation: Biotin. Immunogen: Recombinant osteonectin (Ala18-lle303) expressed in Ecoli. Accession No.: RPA791 Hu01

ncbi gi num :

338325

ncbi acc num :

AAA60993.1

uniprot acc num :

P09486

ncbi mol weight :

34,632 Da

ncbi pathways :

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); ECM Proteoglycans Pathway (833812); Extracellular Matrix Organization Pathway (576262); Hemostasis Pathway (106028); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048); Scavenging By Class H Receptors Pathway (833817); Senescence And Autophagy Pathway (198780); TGF-beta Receptor Signaling Pathway (198774)

ncbi summary :

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]

uniprot summary :

SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 5q31.3-q32. Cellular Component: extracellular space; platelet alpha granule membrane; cell surface; nuclear matrix; mitochondrion; cytoplasm; extracellular region; plasma membrane; basement membrane. Molecular Function: collagen binding; protein binding; extracellular matrix binding; calcium ion binding. Biological Process: response to gravity; platelet activation; receptor-mediated endocytosis; response to peptide hormone stimulus; ossification; extracellular matrix organization and biogenesis; response to cAMP; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; signal transduction; response to L-ascorbic acid; negative regulation of angiogenesis; platelet degranulation; response to cadmium ion; response to ethanol; response to cytokine stimulus; negative regulation of endothelial cell proliferation; response to lead ion; regulation of cell morphogenesis; blood coagulation; response to calcium ion; inner ear development; lung development. Disease: Osteogenesis Imperfecta, Type Xvii

size1 :

0.1 mg

price1 :

355 USD

more info or order :

MyBioSource product webpage