catalog number :
MBS2004903
products full name :
Polyclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8)
products short name :
[Milk Fat Globule EGF Factor 8 (MFGE8)]
other names :
[lactadherin isoform 1; Lactadherin; lactadherin; milk fat globule-EGF factor 8 protein; MFGM; Milk fat globule-EGF factor 8; MFG-E8; SED1; Sperm surface protein SP47; MP47]
products gene name :
[MFGE8]
other gene names :
[Mfge8; Mfge8; P47; MP47; Mfgm; SED1; MFG-E8; AA408458; AI325141; lactadherin; MFG-E8; MP47]
purity :
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
form :
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300,50% glycerol.
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
tested application :
WB; IHC; ICC; IP.
app notes :
Western blotting: 0.5-5 ug/mL;1:200-2000. Immunohistochemistry: 5-20 ug/mL;1:50-200. Immunocytochemistry: 5-20 ug/mL;1:50-200. Optimal working dilutions must be determined by end user
image1 heading :
Western Blot (WB)
image2 heading :
Immunohistochemistry (IHC)
other info1 :
Organism Species: Mus musculus (Mouse). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant MFGE8 (Ile159~Ser417 (Accession # P21956)) expressed in E.coli
other info2 :
Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2053520
ncbi acc num :
NP_032620.2
ncbi gb acc num :
NM_008594.2
uniprot summary :
MFGE8: Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization. Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7 D2 7 44.9 cM. Cellular Component: external side of plasma membrane; extracellular exosome; extracellular matrix; extracellular region; extracellular space; extrinsic component of plasma membrane; membrane. Molecular Function: integrin binding; phosphatidylethanolamine binding; phosphatidylserine binding. Biological Process: angiogenesis; cell adhesion; phagocytosis, engulfment; phagocytosis, recognition; positive regulation of apoptotic cell clearance; positive regulation of phagocytosis; single fertilization