Polyclonal Antibody to Mesothelin (MSLN) | MyBioSource MBS2004878 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Mesothelin (MSLN)

catalog :

MBS2004878

quantity :

0.01 mg

price :

135 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2004878

products type :

Antibody

products full name :

Polyclonal Antibody to Mesothelin (MSLN)

products short name :

Mesothelin (MSLN)

other names :

mesothelin isoform 1 preproprotein; Mesothelin; mesothelin; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor; mesothelin; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor

products gene name :

MSLN

other gene names :

MSLN; MSLN; MPF; SMRP; MPF; MPF

uniprot entry name :

MSLN_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse

sequence length :

622

sequence :

The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- PFTYEQ LSIFKHKLDK TYPQGYPESL IQQLGHFFRY VSPEDIHQWN VTSPDTVKTL LKVSKGQKMN AQAIALVACY LRGGGQLDED MVKALGDIPL SYLCDFSPQD LHSVPSSVMW LVGPQDLDKC SQRHLGLLYQ KACSAFQNVS GLEYFEKIKT FLGGASVKDL RALSQHNVSM DIATFKRLQV DSLVGLSVAE VQKLLGPN.

specificity :

The antibody is a rabbit polyclonal antibody raised against MSLN. It has been selected for its ability to recognize MSLN in immunohistochemical staining andwestern blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:50-400. Immunocytochemistry in formalin fixed cells: 1:50-500. Immunohistochemistry in formalin fixed frozen section: 1:50-500. Immunohistochemistry in paraffin section: 1:00-200. Enzyme-linked Immunosorbent Assay: 1:100-200. Optimal working dilutions must be determined by end user.

other info1 :

Immunogen: MSLN, Mouse. Immunogen Information: Recombinant MSLN (Pro345~Asn548) with two N-terminal Tags, His-tag and GST-tag expresssed in E.coli. Acession No.: RPH615Mu01

other info2 :

Quality Control: Content: The quality control contains recombinant CRP (Phe17~Pro224) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

ncbi gi num :

293651530

ncbi acc num :

NP_001170826.1

ncbi gb acc num :

NM_001177355.1

uniprot acc num :

Q13421

ncbi mol weight :

68,986 Da

ncbi summary :

This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]

uniprot summary :

MSLN: Membrane-anchored forms may play a role in cellular adhesion. Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. Belongs to the mesothelin family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: Golgi apparatus; extracellular space; cell surface; membrane; plasma membrane. Molecular Function: protein binding. Biological Process: pancreas development; cell adhesion

size1 :

0.01 mg

price1 :

135 USD

size2 :

0.05 mg

price2 :

245

size3 :

0.1 mg

price3 :

325

more info or order :

MyBioSource product webpage