Polyclonal Antibody to Lymphotoxin Beta (LTb) | MyBioSource MBS2004857 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Lymphotoxin Beta (LTb)

catalog :

MBS2004857

quantity :

0.01 mg

price :

135 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2004857

products type :

Antibody

products full name :

Polyclonal Antibody to Lymphotoxin Beta (LTb)

products short name :

Lymphotoxin Beta (LTb)

other names :

lymphotoxin beta; Lymphotoxin-beta; lymphotoxin-beta; TNF-C; LT-beta; tumor necrosis factor C; tumor necrosis factor ligand superfamily member 3; lymphotoxin beta (TNF superfamily, member 3); Tumor necrosis factor C; TNF-C; Tumor necrosis factor ligand superfamily member 3

products gene name :

LTb

other gene names :

LTB; LTB; p33; TNFC; TNFSF3; TNFC; TNFSF3; LT-beta; TNF-C

uniprot entry name :

TNFC_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse

sequence length :

244

sequence :

The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF-IIG SGAQAQKRLD DSKPSCILPS PSSLSETPDP RLHPQRSNAS RNLASTSQGP VAQSSREASA WMTILSPAAD STPDPGVQQL PKGEPETDLN PELPAAHLIG AWMSGQGLSW EASQEEAFLR SGAQFSPTHG LALPQDGVYY LYCHVGYRGR TPPAGRSRAR SLTLRSALYR AGGAYGRGSP ELLLEGAETV TPVVDPIGYG SLWYTSVGFG GLAQLRSGER VYVNISHPDM VDYRRGKTFF GAVM

specificity :

The antibody is a rabbit polyclonal antibody raised against LTb. It has been selected for its ability to recognize LTb in immunohistochemical staining andwestern blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200

other info1 :

Immunogen: Recombinant LTb (Ile58~Met304) expressed in E.coli.

other info2 :

Quality Control: Content: The quality control contains recombinant Thyroglobulin (Ser2083~Ala2333) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

ncbi gi num :

4337113

ncbi acc num :

AAD18089.1

uniprot acc num :

Q06643

ncbi mol weight :

25,390 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); NF-kappa B Signaling Pathway (634527); Rheumatoid Arthritis Pathway (200309); Rheumatoid Arthritis Pathway (200269); Spinal Cord Injury Pathway (739007)

ncbi summary :

Lymphotoxin beta is a type II membrane protein of the TNF family. It anchors lymphotoxin-alpha to the cell surface through heterotrimer formation. The predominant form on the lymphocyte surface is the lymphotoxin-alpha 1/beta 2 complex (e.g. 1 molecule alpha/2 molecules beta) and this complex is the primary ligand for the lymphotoxin-beta receptor. The minor complex is lymphotoxin-alpha 2/beta 1. LTB is an inducer of the inflammatory response system and involved in normal development of lymphoid tissue. Lymphotoxin-beta isoform b is unable to complex with lymphotoxin-alpha suggesting a function for lymphotoxin-beta which is independent of lympyhotoxin-alpha. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

uniprot summary :

LTB: Cytokine that binds to LTBR/TNFRSF3. May play a specific role in immune response regulation. Provides the membrane anchor for the attachment of the heterotrimeric complex to the cell surface. Isoform 2 is probably non-functional. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: extracellular space; integral to membrane. Molecular Function: cytokine activity; tumor necrosis factor receptor binding; receptor binding. Biological Process: skin development; positive regulation of interleukin-12 biosynthetic process; cell-cell signaling; immune response; gene expression; signal transduction; lymph node development

size1 :

0.01 mg

price1 :

135 USD

size2 :

0.05 mg

price2 :

230

size3 :

0.1 mg

price3 :

310

more info or order :

MyBioSource product webpage