product summary
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company name :
MyBioSource
product type :
antibody
product name :
Polyclonal Antibody to Fibronectin (FN)
catalog :
MBS2004823
quantity :
0.01 mg
price :
135 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
Xenopus laevis
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS2004823
products type :
Antibody
products full name :
Polyclonal Antibody to Fibronectin (FN)
products short name :
Fibronectin (FN)
other names :
fibronectin; Fibronectin; fibronectin; FN; gene C; fibronectin 1<
products gene name :
FN
other gene names :
fn1; fn1; fibronectin; FN
uniprot entry name :
FINC_XENLA
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Rat
sequence length :
2481
sequence :
The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGS- SNGEPC VLPFHYNGRT FYSCTTEGRQ DGHLWCSTTS NYEQDQKYSF CTDHAVLVQT RGGNSNGALC HFPFLYSNRN YSDCTSEGRR DNMKWCGTTQ NYDADQKFGF CPMAAHEEIC TTNEGVMYRI GDQWDKQHDL GHMMRCTCVG NGRGQWACIP YSQLRDQCIV DDITYNVNDT FHKRHEEGHM LNCTCFGQGR GRWKCDPI
specificity :
The antibody is a rabbit polyclonal antibody raised against FN. It has been selected for its ability to recognize FN in immunohistochemical staining andwestern blotting.
purity :
Affinity Chromatography
form :
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
concentration :
200ug/ml
storage stability :
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
tested application :
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
app notes :
Western blotting: 1:50-400. Immunocytochemistry in formalin fixed cells: 1:50-500. Immunohistochemistry in formalin fixed frozen section: 1:50-500. Immunohistochemistry in paraffin section: 1:10-100. Enzyme-linked Immunosorbent Assay: 1:100-200. Optimal working dilutions must be determined by end user.
other info1 :
Immunogen: FN, Rat . Immunogen Information: Recombinant FN (Ser355~Ile558) with N-terminal His-Tag expressed in E.coli. Accession No.: RPA037Ra01. UOM: 100ug
other info2 :
Quality Control: Content: The quality control contains recombinant FN (Gly2117~Leu2236) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
ncbi gi num :
148237139
ncbi acc num :
NP_001081270.1
ncbi gb acc num :
NM_001087801.1
uniprot acc num :
Q91740
ncbi mol weight :
272,680 Da
ncbi pathways :
ECM-receptor Interaction Pathway (119288); ECM-receptor Interaction Pathway (479); Focal Adhesion Pathway (119287); Focal Adhesion Pathway (478); Regulation Of Actin Cytoskeleton Pathway (119294); Regulation Of Actin Cytoskeleton Pathway (500)
uniprot summary :
Function: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. May be involved in osteoblast compaction . By similarity. Subunit structure: Dimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends . By similarity. Tissue specificity: In early Xenopus embryo, cellular forms of fibronectin predominate which include both extra domains. In fibronectin of embryonic and adult liver the connecting strand 3 can be either completely excluded or included. Post-translational modification: Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers). Sequence similarities: Contains 12 fibronectin type-I domains.Contains 2 fibronectin type-II domains.Contains 17 fibronectin type-III domains.
size1 :
0.01 mg
price1 :
135 USD
size2 :
0.05 mg
price2 :
230
size3 :
0.1 mg
price3 :
310
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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