catalog number :
MBS2004778
products full name :
Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)
products short name :
Brain Derived Neurotrophic Factor (BDNF)
other names :
brain derived neurotrophic factor; Brain-derived neurotrophic factor; brain-derived neurotrophic factor; pre-pro-brain-derived; brain-derived neurotrophic factor
products gene name :
BDNF
other gene names :
BDNF; BDNF; BDNF
uniprot entry name :
BDNF_CHICK
sequence :
The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF-MVISY FGCMKAAPMK EANVRGQGSL AYPGMRTHGT LESVNGPKTG SRGLTSLADT FEHVIEELLD EDQKVRPNEE NNKDADLYTS RVMLSSQVPL EPPLLFLLEE YKNYLDAANM SMRVRRHSDP ARRGELSVCD SISEWVTAAD KKTAVDMSGG TVTVLEKVPV SKG
specificity :
The antibody is a cavia polyclonal antibody raised against BDNF. It has been selected for its ability to recognize BDNF in immunohistochemical staining and western blotting.
purity :
Affinity Chromatography
form :
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
storage stability :
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
tested application :
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
app notes :
Western blotting: 1:50-400. Immunocytochemistry in formalin fixed cells: 1:50-500. Immunohistochemistry in formalin fixed frozen section: 1:50-500. Immunohistochemistry in paraffin section: 1:10-100. Enzyme-linked Immunosorbent Assay: 1:100-200. Optimal working dilutions must be determined by end user.
other info1 :
Immunogen: BDNF, Rabbit. Immunogen Information: Recombinant BDNF (Met16~Gly183) with N-terminal His-Tagexpressed in E.coli. Accession No.: RPA011Rb01. UOM: 100ug
other info2 :
Quality Control: Content: The quality control contains recombinant CRP (Phe17~Pro224) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
ncbi acc num :
AAZ31074.1
ncbi mol weight :
27,715 Da
ncbi pathways :
MAPK Signaling Pathway (199082); MAPK Signaling Pathway (83956); MAPK Signaling Pathway (456)
uniprot summary :
Function: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Subcellular location: Secreted. Sequence similarities: Belongs to the NGF-beta family.