Polyclonal Antibody to Phenylalanine Hydroxylase (PAH) | MyBioSource MBS2004517 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Phenylalanine Hydroxylase (PAH)

catalog :

MBS2004517

quantity :

0.01 mg

price :

110 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Lane1: Human Liver Tissue; Lane2: Porcine Kidney Tissue; Lane3: Mouse Gallbladder Tissue; Lane4: Human Hela Cells.

image 2 :

Western Blot: Sample: Recombinant PAH, Human.

image 3 :

DAB staining on IHC-P; Samples: Human Kidney Tissue

product information

catalog number :

MBS2004517

products type :

Antibody

products full name :

Polyclonal Antibody to Phenylalanine Hydroxylase (PAH)

products short name :

[Phenylalanine Hydroxylase (PAH)]

other names :

[phenylalanine-4-hydroxylase; Phenylalanine-4-hydroxylase; phenylalanine-4-hydroxylase; phenylalanine hydroxylase; Phe-4-monooxygenase]

products gene name :

[PAH]

other gene names :

[PAH; PAH; PH; PKU; PKU1; PAH]

clonality :

Polyclonal

host :

Rabbit

purity :

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

form :

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

concentration :

1 mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP).

app notes :

Western blotting: 0.5-2 ug/mL;1:500-2000. Immunohistochemistry: 5-20 ug/mL;1:50-200. Immunocytochemistry: 5-20 ug/mL;1:50-200. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

DAB staining on IHC-P; Samples: Human Kidney Tissue.

image5 heading :

Knockout Validation

image5 description :

Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: PAH knockout Hela cell lysate; Predicted MW: 51kd Observed MW: 55kd Primary Ab: 2ug/ml Rabbit Anti-Human PAH Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)

other info1 :

Organism Species: Homo sapiens (Human). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant PAH (Met1~Gln226) expressed in E.coli

other info2 :

Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2067989

products categories :

Knock-Out Validation Antibody

ncbi gi num :

4557819

ncbi acc num :

NP_000268.1

ncbi gb acc num :

NM_000277.2

uniprot acc num :

P00439

ncbi pathways :

Abnormal Metabolism In Phenylketonuria Pathway (645287); Biogenic Amine Synthesis Pathway (198793); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Disease Pathway (530764); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Phenylalanine And Tyrosine Catabolism Pathway (106189); Phenylalanine Metabolism Pathway (82960)

ncbi summary :

This gene encodes a member of the biopterin-dependent aromatic amino acid hydroxylase protein family. The encoded phenylalanine hydroxylase enzyme hydroxylates phenylalanine to tyrosine and is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria. [provided by RefSeq, Aug 2017]

uniprot summary :

PAH: phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria. Protein type: Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; EC 1.14.16.1; Oxidoreductase. Chromosomal Location of Human Ortholog: 12q23.2. Cellular Component: cytosol; extracellular exosome. Molecular Function: iron ion binding; phenylalanine 4-monooxygenase activity. Biological Process: amino acid biosynthetic process; catecholamine biosynthetic process; L-phenylalanine catabolic process; neurotransmitter biosynthetic process. Disease: Phenylketonuria

size1 :

0.01 mg

price1 :

110 USD

size2 :

0.02 mg

price2 :

130

size3 :

0.05 mg

price3 :

175

size4 :

0.1 mg

price4 :

225

size5 :

0.2 mg

price5 :

340

size6 :

1 mg

price6 :

800

more info or order :

MyBioSource product webpage