Polyclonal Antibody to A Disintegrin And Metalloprotease 10 (ADAM10) | MyBioSource MBS2004491 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to A Disintegrin And Metalloprotease 10 (ADAM10)

catalog :

MBS2004491

quantity :

0.01 mg

price :

135 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2004491

products type :

Antibody

products full name :

Polyclonal Antibody to A Disintegrin And Metalloprotease 10 (ADAM10)

products short name :

A Disintegrin And Metalloprotease 10 (ADAM10)

other names :

A disintegrin and metalloproteinase with thrombospondin motifs 10 isoform 1 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 10; A disintegrin and metalloproteinase with thrombospondin motifs 10; ADAM-TS 10; zinc metalloendopeptidase; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 10; ADAM metallopeptidase with thrombospondin type 1 motif, 10

products gene name :

ADAM10

other gene names :

ADAMTS10; ADAMTS10; WMS; WMS1; ADAM-TS10; ADAMTS-10; ADAM-TS 10; ADAM-TS10; ADAMTS-10

uniprot entry name :

ATS10_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Rat

sequence length :

1103

sequence :

The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF-TSG DKLNNNKFSL CSIRNISQVL EKKRNNCFVE SGQPICGNGM VEQGEECDCG YSDQCKDECC FDANQPEGKK CKLKPGKQCS PSQGPCCTAQ CAFKSKSEKC RDDSDCAKEG ICNGFTALCP ASDPKPNFTD CNRHTQVCIN GQCAGSICEK YDLEECTCAS SDGKDDKELC HVCCMKKMAP STCASTGSLQ WNKQFTGRTI TLQPGSPCND FRGYCDVFMR CRLVD

specificity :

The antibody is a rabbit polyclonal antibody raised against ADAM10. It has been selected for its ability to recognize ADAM10 in immunohistochemical staining andwestern blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200

other info1 :

Immunogen: Recombinant ADAM10 (Thr218~Asp445) expressed in E.coli.

other info2 :

Quality Control: Content: The quality control contains recombinant Thyroglobulin (Ser2083~Ala2333) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

ncbi gi num :

537544566

ncbi acc num :

NP_112219.3

ncbi gb acc num :

NM_030957.3

uniprot acc num :

Q9H324

ncbi mol weight :

120,874 Da

ncbi summary :

This gene belongs to the ADAMTS (a disintegrin and metalloproteinase domain with thrombospondin type-1 motifs) family of zinc-dependent proteases. ADAMTS proteases are complex secreted enzymes containing a prometalloprotease domain of the reprolysin type attached to an ancillary domain with a highly conserved structure that includes at least one thrombospondin type 1 repeat. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration. The product of this gene plays a major role in growth and in skin, lens, and heart development. It is also a candidate gene for autosomal recessive Weill-Marchesani syndrome. [provided by RefSeq, Jul 2008]

uniprot summary :

ADAMTS10: Metalloprotease that participate in microfibrils assembly. Microfibrils are extracellular matrix components occurring independently or along with elastin in the formation of elastic tissues. Defects in ADAMTS10 are the cause of Weill-Marchesani syndrome 1 (WMS1). WMS1 is a rare connective tissue disorder characterized by short stature, brachydactyly, joint stiffness, and eye abnormalities including microspherophakia, ectopia lentis, severe myopia and glaucoma. Protein type: Motility/polarity/chemotaxis; Protease; EC 3.4.24.-; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: extracellular matrix; microfibril. Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity. Biological Process: proteolysis. Disease: Weill-marchesani Syndrome 1

size1 :

0.01 mg

price1 :

135 USD

size2 :

0.05 mg

price2 :

230

size3 :

0.1 mg

price3 :

310

more info or order :

MyBioSource product webpage