Polyclonal Antibody to High Molecular Weight Kininogen (HMWK) | MyBioSource MBS2004449 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to High Molecular Weight Kininogen (HMWK)

catalog :

MBS2004449

quantity :

0.01 mg

price :

140 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2004449

products type :

Antibody

products full name :

Polyclonal Antibody to High Molecular Weight Kininogen (HMWK)

products short name :

High Molecular Weight Kininogen (HMWK)

other names :

high molecular weight kininogen I isoform DeltaD5; Kininogen-1; kininogen-1; H-kininigen; L-kininogen; kininogen 1

products gene name :

HMWK

other gene names :

Kng1; Kng1; Kng; Kng

uniprot entry name :

KNG1_MOUSE

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Rat

sequence length :

480

sequence :

The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-LHEDCVP LPSGDDGECK GNAFVDIHKT IAGFSDSCEF YPGDDLFELL PEDCPGCPRN IPVDSPELKE ALGHSIAQLN AENNHTFYFK IDTVKKATSQ VVAGTKYVIE FIARETKCSK ESNAELTADC ETKRLGQSLN CNANVYMRPW ENKVVPTVKC KVLDMTSVIR RPPGFSPFRA PRVKKPKEST TVSPSYIARV QEERDPGNEQ GPIHGHGWLH AKQIKNKNHQ GHKHGHGIGH GHQKPHGLGH GHQLKLDDLK QQRED

specificity :

The antibody is a rabbit polyclonal antibody raised against HMWK. It has been selected for its ability to recognize HMWK in immunohistochemical staining and western blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:50-400. Immunocytochemistry in formalin fixed cells: 1:50-500. Immunohistochemistry in formalin fixed frozen section: 1:50-500. Immunohistochemistry in paraffin section: 1:10-100. Enzyme-linked Immunosorbent Assay: 1:100-200. Optimal working dilutions must be determined by end user.

ncbi gi num :

50082914

ncbi acc num :

AAT70087.1

uniprot acc num :

O08677

ncbi mol weight :

59kDa

ncbi pathways :

ACE Inhibitor Pathway (198320); Class A/1 (Rhodopsin-like Receptors) Pathway (926991); Complement And Coagulation Cascades Pathway (198335); Complement And Coagulation Cascades Pathway (83270); Complement And Coagulation Cascades Pathway (484); Formation Of Fibrin Clot (Clotting Cascade) Pathway (925989); G Alpha (i) Signalling Events Pathway (927022); G Alpha (q) Signalling Events Pathway (927024); GPCR Downstream Signaling Pathway (927020); GPCR Ligand Binding Pathway (926990)

uniprot summary :

KNG1: (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin- induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW- kininogen is in contrast to HMW-kininogen not involved in blood clotting. Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency). HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Cell adhesion; Contractile; Inhibitor. Cellular Component: extracellular space; extracellular region. Molecular Function: protease inhibitor activity; cysteine protease inhibitor activity; receptor binding. Biological Process: hemostasis; negative regulation of peptidase activity; inflammatory response; regulation of blood vessel size; blood coagulation; vasodilation

size1 :

0.01 mg

price1 :

140 USD

size2 :

0.05 mg

price2 :

245

size3 :

0.1 mg

price3 :

330

more info or order :

MyBioSource product webpage