14-3-3 epsilon antibody | MyBioSource MBS200428 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

14-3-3 epsilon antibody

catalog :

MBS200428

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[AT4F8]

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 epsilon antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate Lane 3.: 293T cell lysate Lane 4.: A549 cell lysate Lane 5.: Mouse Brain Tissue lysate The Recombinant Protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 epsilon antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 14-3-3 Zeta Recombinant Protein Lane 2.: 14-3-3 Beta Recombinant Protein Lane 3.: 14-3-3 Epsilon Recombinant Protein Lane 4.: 14-3-3 Eta Recombinant Protein Lane 5.: 14-3-3 Gamma Recombinant Protein Lane 6.: 14-3-3 Sigma Recombinant Protein Lane 7.: 14-3-3 Tau Recombinant Protein

image 2 :

ICC/IF analysis of 14-3-3 epsilon in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 epsilon antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of 14-3-3 epsilon in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 epsilon antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

product information

catalog number :

MBS200428

products type :

Antibody

products full name :

14-3-3 epsilon antibody

products short name :

[14-3-3 epsilon]

products name syn :

[Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein epsilon polypeptide; YWHAE; MDS; MDCR; KCIP-1; FLJ45465; 14-3-3 epsilon; Tyrosine 3/tryptophan 5 -monooxygenase activation protein; epsilon polypeptide; Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein; epsilon polypeptide 14 3 3 E; 14 3 3E; KCIP 1; KCIP1; Mitochondrial import stimulation factor L subunit; Protein kinase C inhibitor protein1; Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide.]

other names :

[14-3-3 protein epsilon; 14-3-3 protein epsilon; 14-3-3 protein epsilon; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon]

products gene name :

[14-3-3 epsilon]

other gene names :

[YWHAE; YWHAE; MDS; HEL2; MDCR; KCIP-1; 14-3-3E; 14-3-3E]

clonality :

Monoclonal

isotype :

IgG2b, k

clone :

[AT4F8]

host :

Mouse

reactivity :

Human

purity :

By protein-A affinity chromatography

form :

Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.

concentration :

1 mg/ml

storage stability :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20 C to -80C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

app notes :

Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: Recombinant human14-3-3 epsilon (1-255aa) purified from E. coli

products categories :

Neuroscience

products references :

Oriente F., et al. (2005) J Biol Chem. 280 (49): 40642-9.; Conklin D., et al. (1995) PNAS. 92 (17): 7892-6.

ncbi gi num :

5803225

ncbi acc num :

NP_006752

ncbi gb acc num :

NM_006761.4

uniprot acc num :

P62258

ncbi pathways :

AURKA Activation By TPX2 Pathway (1383018); Activation Of BAD And Translocation To Mitochondria Pathway (1270271); Activation Of BH3-only Proteins Pathway (1270270); Anchoring Of The Basal Body To The Plasma Membrane Pathway (1268847); Apoptosis Pathway (1270262); Calcium Regulation In The Cardiac Cell Pathway (198906); Cell Cycle Pathway (198811); Cell Cycle Pathway (1269741); Cell Cycle Checkpoints Pathway (1269742); Cell Cycle, Mitotic Pathway (1269763)

ncbi summary :

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]

uniprot summary :

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326).

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage