antibody

Biotin-Linked Antibody to A Disintegrin And Metalloprotease 17 (ADAM17)

MBS2004262

0.1 mg

335 USD

polyclonal

rabbit

biotin

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Biotin-Linked Antibody to A Disintegrin And Metalloprotease 17 (ADAM17)

A Disintegrin And Metalloprotease 17 (ADAM17)

A disintegrin and metalloproteinase with thrombospondin motifs 17 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 17; A disintegrin and metalloproteinase with thrombospondin motifs 17; ADAM-TS17; ADAMTS-17; ADAM-TS 17; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 17; ADAM metallopeptidase with thrombospondin type 1 motif, 17

ADAM17

ADAMTS17; ADAMTS17; ADAM-TS 17; ADAM-TS17; ADAMTS-17

ATS17_HUMAN

Polyclonal

IgG

Rabbit

Human

1095

The antibody is a rabbit polyclonal antibody raised against ADAM17 conjugated to biotin. It has been selected for its ability to recognize ADAM17 in immunohistochemical staining andwestern blotting.

Affinity Chromatography

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

200ug/ml

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)

Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200

Conjugation: Biotin

Biotin Antibody

110611170

NP_620688.2

NM_139057.2

Q8TE56

121,127 Da

Subcellular location: Secreted extracellular space extracellular matrix . By similarity. Tissue specificity: Isoform 1 and isoform 2 are expressed at high levels in the lung, brain, whole eye and retina. Isoform 1 shows a weaker expression in the heart, kidney and skeletal muscle. Isoform 2 shows a weaker expression in the kidney, bone marrow and skeletal muscle. Isoform 1 and isoform 2 are expressed at high levels in the fetal heart, kidney, and whole eye, whereas a weak expression is seen in the fetal liver. Ref.5. Domain: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. Post-translational modification: The precursor is cleaved by a furin endopeptidase . By similarity.Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion . By similarity. Involvement in disease: Weill-Marchesani-like syndrome (WMLS) [MIM:613195]: A disorder characterized by many of the key features of Weill-Marchesani syndrome, including lenticular myopia, ectopia lentis, glaucoma, spherophakia and short stature. However, the characteristic brachydactyly or decreased joint flexibility of Weill-Marchesani syndrome are absent.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.5. Sequence similarities: Contains 1 disintegrin domain.Contains 1 peptidase M12B domain.Contains 1 PLAC domain.Contains 5 TSP type-1 domains.

0.1 mg

335 USD