catalog number :
MBS2004259
products full name :
Polyclonal Antibody to Carbonic Anhydrase IX (CA9)
products short name :
[Carbonic Anhydrase IX (CA9)]
other names :
[carbonic anhydrase 9; Carbonic anhydrase 9; carbonic anhydrase 9; carbonic anhydrase 9; Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN homolog]
products gene name :
[CA9]
other gene names :
[Car9; Ca9; Ca9; CAIX; MN/CA9; Car9; CA-IX; CAIX]
specificity :
The antibody is a Rabbit polyclonal antibody raised against CA9. It has been selected for its ability to recognize CA9 in immunohistochemical staining and western blotting.
purity :
Antigen-specific Affinity Chromatography.
form :
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
tested application :
WB; IHC; ICC; IP
app notes :
Western blotting: 0.5-2µg/mL. Immunohistochemistry: 5-20µg/mL. Immunocytochemistry: 5-20µg/mL. Optimal working dilutions must be determined by end user
image1 heading :
Western Blot (WB)
image2 heading :
Immunohistochemistry (IHC)
other info1 :
Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant CA9 (Glu88~Glu280) expressed in E.coli
other info2 :
Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2069862
ncbi acc num :
NP_647466.2
ncbi gb acc num :
NM_139305.2
ncbi mol weight :
30,247 Da
ncbi pathways :
Metabolism Pathway (1367614); Nitrogen Metabolism Pathway (83222); Nitrogen Metabolism Pathway (416); Reversible Hydration Of Carbon Dioxide Pathway (1367894)
uniprot summary :
CA9: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Forms oligomers linked by disulfide bonds. By hypoxia. Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). Belongs to the alpha-carbonic anhydrase family. Protein type: EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase; Membrane protein, integral; Nucleolus. Chromosomal Location of Human Ortholog: 4 4 A5. Cellular Component: basolateral plasma membrane; cell projection; integral component of membrane; membrane; plasma membrane. Molecular Function: carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding. Biological Process: morphogenesis of an epithelium; one-carbon metabolic process; secretion