Polyclonal Antibody to Deoxyribonuclease I (DNASE1) | MyBioSource MBS2004226 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Deoxyribonuclease I (DNASE1)

catalog :

MBS2004226

quantity :

0.01 mg

price :

105 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Recombinant protein.

image 2 :

DAB staining on IHC-P; Samples: Human Liver Tissue

image 3 :

DAB staining on IHC-P; Samples: Human Liver Cancer Tissue.

product information

catalog number :

MBS2004226

products type :

Antibody

products full name :

Polyclonal Antibody to Deoxyribonuclease I (DNASE1)

products short name :

[Deoxyribonuclease I (DNASE1)]

other names :

[deoxyribonuclease-1; Deoxyribonuclease-1; deoxyribonuclease-1; deoxyribonuclease 1; Deoxyribonuclease I; DNase I; INN: Dornase alfa]

products gene name :

[DNASE1]

other gene names :

[DNASE1; DNASE1; DNL1; DRNI; DNL1; DRNI; DNase I]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

purity :

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

form :

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

concentration :

1mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

WB; IHC; ICC; IP.

app notes :

Western blotting: 0.5-2ug/mL;1:500-2000. Immunohistochemistry: 5-20ug/mL;1:50-200. Immunocytochemistry: 5-20ug/mL;1:50-200. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Knockout Validation

image4 description :

Knockout Validation: Lane 1: Wild-type 293T cell lysate; Lane 2: DNASE1 knockout 293T cell lysate; Predicted MW: 20,31kDa Observed MW: 20kDa Primary Ab: 2ug/ml Rabbit Anti-Human DNASE1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)

other info1 :

Organism Species: Homo sapiens (Human). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant DNASE1 (Gly19~Ala259) expressed in E.coli

other info2 :

Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2051801

products categories :

Knock-Out Validation Antibody

ncbi gi num :

21361254

ncbi acc num :

NP_005214.2

ncbi gb acc num :

NM_005223.3

uniprot acc num :

P24855

ncbi summary :

This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

uniprot summary :

DNASE1: Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Defects in DNASE1 are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the DNase I family. Protein type: Deoxyribonuclease; EC 3.1.21.1; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: extracellular region; nuclear envelope; nucleus. Molecular Function: actin binding; deoxyribonuclease I activity; protein binding. Biological Process: apoptosis; DNA catabolic process. Disease: Systemic Lupus Erythematosus

size1 :

0.01 mg

price1 :

105 USD

size2 :

0.02 mg

price2 :

125

size3 :

0.05 mg

price3 :

170

size4 :

0.1 mg

price4 :

220

size5 :

0.2 mg

price5 :

320

size6 :

1 mg

price6 :

760

more info or order :

MyBioSource product webpage