Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4) | MyBioSource MBS2004212 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4)

catalog :

MBS2004212

quantity :

0.05 mg

price :

295 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2004212

products type :

Antibody

products full name :

Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4)

products short name :

A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4)

other names :

A disintegrin and metalloproteinase with thrombospondin motifs 4 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 4; A disintegrin and metalloproteinase with thrombospondin motifs 4; ADAM-TS4; ADAM-TS 4; aggrecanase-1; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4; ADAM metallopeptidase with thrombospondin type 1 motif, 4; ADMP-1; Aggrecanase-1

products gene name :

ADAMTS4

other gene names :

ADAMTS4; ADAMTS4; ADMP-1; ADAMTS-2; ADAMTS-4; KIAA0688; ADAM-TS 4; ADAM-TS4; ADAMTS-4

uniprot entry name :

ATS4_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

837

sequence :

The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSGSEF-TFPG KDYDADRQCQ LTFGPDSRHC PQLPPPCAAL WCSGHLNGHA MCQTKHSPWA DGTPCGPAQA CMGGRCLHMD QLQDFNIPQA GGWGPWGPWG DCSRTCGGGV QFSSRDCTRP VPRNGGKYCE GRRTRFRSCN TEDCP

specificity :

The antibody is a rabbit polyclonal antibody raised against ADAMTS4. It has been selected for its ability to recognize ADAMTS4 in immunohistochemical staining andwestern blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200

other info1 :

Immunogen: Recombinant ADAMTS4 (Thr437~Pro575) expressed in E.coli.

other info2 :

Quality Control: Content: The quality control contains recombinant CRP (Phe17~Pro224) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

ncbi gi num :

157427675

ncbi acc num :

NP_005090.3

ncbi gb acc num :

NM_005099.4

uniprot acc num :

O75173

ncbi mol weight :

90,197 Da

ncbi pathways :

Degradation Of The Extracellular Matrix Pathway 576263!!Degradation Of The Extracellular Matrix Pathway 934113!!Endochondral Ossification Pathway 198812!!Extracellular Matrix Organization Pathway 576262!!Extracellular Matrix Organization Pathway 934100

uniprot summary :

Function: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu- -Ala-393' site. Catalytic activity: Glutamyl endopeptidase; bonds cleaved include 370-Thr-Glu-Gly-Glu- -Ala-Arg-Gly-Ser-377 in the interglobular domain of mammalian aggrecan. Cofactor: Binds 1 zinc ion per subunit. Ref.9. Subunit structure: Interacts with SRPX2. Ref.8. Subcellular location: Secreted extracellular space extracellular matrix . By similarity. Tissue specificity: Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles. Induction: By IL1/interleukin-1. Domain: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. Post-translational modification: The precursor is cleaved by a furin endopeptidase.Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion . By similarity. Sequence similarities: Contains 1 disintegrin domain.Contains 1 peptidase M12B domain.Contains 1 TSP type-1 domain. Caution: Has sometimes been referred to as ADAMTS2. Sequence caution: The sequence BAA31663.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

size :

0.05 mg

price :

295 USD

more info or order :

MyBioSource product webpage