Polyclonal Antibody to Tryptase (TPS) | MyBioSource MBS2004125 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Tryptase (TPS)

catalog :

MBS2004125

quantity :

0.01 mg

price :

145 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2004125

products type :

Antibody

products full name :

Polyclonal Antibody to Tryptase (TPS)

products short name :

Tryptase (TPS)

other names :

tryptase; Tryptase alpha/beta-1; tryptase alpha/beta-1; tryptase I; tryptase-1; tryptase-I; tryptase-III; tryptase beta 1; tryptase beta I; tryptase beta-1; tryptase alpha-1; tryptase alpha II; mast cell beta I tryptase; mast cell alpha II tryptase; tryptase alpha/beta 1; Tryptase I; Tryptase alpha-1

products gene name :

TPS

other gene names :

TPSAB1; TPSAB1; TPS1; TPS2; TPSB1; TPS1; TPS2; TPSB1; Tryptase-1

uniprot entry name :

TRYB1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Porcine

sequence length :

275

sequence :

The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD WLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-KGTRCWVTGW GDVHSGWPLP PPYPLKQVRV PIVENSECDM QYHLGLSTGD NIPIVRDDML CAGSEGHDSC QGDSGGPLVC RVNGTWLQAG WSWGEGCAL. PNRPGIYTRV THYLDWIHQC IPRES

specificity :

The antibody is a rabbit polyclonal antibody raised against TPS. It has been selected for its ability to recognize TPS in immunohistochemical staining andwestern blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml. UOM: 100IJg

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200

other info1 :

Immunogen: TPS, Porcine

other info2 :

Quality Control: Content: The quality control contains recombinant TPS(Lys151~Ser275) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

ncbi gi num :

1182067

ncbi acc num :

AAA86934.1

uniprot acc num :

Q15661

ncbi mol weight :

30,515 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262)

ncbi summary :

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

uniprot summary :

TPSAB1: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Belongs to the peptidase S1 family. Tryptase subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Protease; EC 3.4.21.59; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: extracellular matrix; extracellular space; extracellular region. Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity. Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; defense response; proteolysis

size1 :

0.01 mg

price1 :

145 USD

size2 :

0.05 mg

price2 :

260

size3 :

0.1 mg

price3 :

360

more info or order :

MyBioSource product webpage