Polyclonal Antibody to Decay Accelerating Factor (DAF) | MyBioSource MBS2004075 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Decay Accelerating Factor (DAF)

catalog :

MBS2004075

quantity :

0.01 mg

price :

105 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Mouse Heart Tissue.

image 2 :

Western Blot: Sample: Recombinant DAF, Mouse.

image 3 :

DAB staining on IHC-P; Samples: Mouse Kidney Tissue.

product information

catalog number :

MBS2004075

products type :

Antibody

products full name :

Polyclonal Antibody to Decay Accelerating Factor (DAF)

products short name :

[Decay Accelerating Factor (DAF)]

other names :

[complement decay-accelerating factor, GPI-anchored preproprotein; Complement decay-accelerating factor, GPI-anchored; complement decay-accelerating factor, GPI-anchored; CD55 molecule, decay accelerating factor for complement; CD_antigen: CD55]

products gene name :

[DAF]

other gene names :

[Cd55; Cd55; Daf; Daf1; Daf-GPI; GPI-DAF; Cd55a; Daf; Daf1; DAF-GPI]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mus musculus (Mouse)

sequence length :

sequence :

Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF- PILG RHSKFAEQSK VAYSCNNGFK QVPDKSNIVV CLENGQWSSH ETFCEKSCVA PERLSFASLK KEYLNMNFFP VGTIVEYECR PGFRKQPPLP GKATCLEDLV WSPVAQFCKK KSCPNPKDLD NGHINIPTGI LFGSEINFSC NPGYRLVGVS STFCSVTGNT VDWDDEFPVC TEIHCPEPPK INNGIMRGES DSYTYSQVVT YSCDKGFILV GNASIYCTVS KSDVGQWSSP PPRCIE

specificity :

The antibody is a rabbit polyclonal antibody raised against DAF. It has been selected for its ability to recognize DAF in immunohistochemical staining and western blotting.

purity :

Antigen-specific Affinity Chromatography.

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4oC for frequent use. Aliquot and store at -20oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.

app notes :

Western blotting: 0.5-2ug/ml. Immunocytochemistry in formalin fixed cells: 5-20ug/ml. Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml. Immunohistochemistry in paraffin section: 5-20ug/ml. Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

DAB staining on IHC-P; Samples: Mouse Stomach Tissue

image5 heading :

Immunohistochemistry (IHC)

image5 description :

DAB staining on IHC-P; Samples: Mouse Spleen Tissue

other info1 :

Source: Polyclonal antibody preparation. Traits: Liquid

other info2 :

Immunogen: Recombinant DAF (Pro47~Glu286) expressed in E.coli. Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2042690

ncbi gi num :

114326522

ncbi acc num :

NP_034146.2

ncbi gb acc num :

NM_010016.3

uniprot acc num :

Q61475

ncbi mol weight :

42,618 Da

ncbi pathways :

Allograft Rejection Pathway (920963); Asparagine N-linked Glycosylation Pathway (1367240); COPI-mediated Anterograde Transport Pathway (1367178); Class B/2 (Secretin Family Receptors) Pathway (1367080); Complement Activation, Classical Pathway (198379); Complement And Coagulation Cascades Pathway (198335); Complement And Coagulation Cascades Pathway (83270); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (1367536); ER To Golgi Anterograde Transport Pathway (1367175)

ncbi summary :

This gene encodes an inhibitor of both the classical and the alternative pathways of complement activation. The encoded preproprotein undergoes post-translational processing to generate a mature polypeptide anchored to the plasma membrane via a glycosylphosphatidylinositol moiety. Erythrocytes from mice deficient in the encoded protein exhibit impaired regulation of complement activation resulting in enhanced complement deposition. Mice lacking the encoded protein exhibit enhanced susceptibility to experimentally induced myasthenia gravis. This gene is located adjacent to a closely related gene on chromosome 1. [provided by RefSeq, Nov 2015]

uniprot summary :

CD55: This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Belongs to the receptors of complement activation (RCA) family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell surface; Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 1 E4 1 56.89 cM. Cellular Component: apical plasma membrane; cell surface; external side of plasma membrane; membrane; membrane raft; plasma membrane. Molecular Function: enzyme inhibitor activity; lipid binding; viral receptor activity. Biological Process: complement activation, classical pathway; immune system process; innate immune response; regulation of complement activation, classical pathway

size1 :

0.01 mg

price1 :

105 USD

size2 :

0.02 mg

price2 :

125

size3 :

0.05 mg

price3 :

170

size4 :

0.1 mg

price4 :

210

size5 :

0.2 mg

price5 :

315

size6 :

1 mg

price6 :

745

more info or order :

MyBioSource product webpage