Polyclonal Antibody to Titin (TTN) | MyBioSource MBS2004003 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Titin (TTN)

catalog :

MBS2004003

quantity :

0.01 mg

price :

105 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Figure. Western Blot; Sample: Recombinant TTN, Human.

image 2 :

DAB staining on IHC-P; Samples: Human Skeletal muscle Tissue;. Primary Ab: 208g/ml Rabbit Anti- Human TTN Antibody. Second Ab: 28g/mL HRP-Linked Caprine Anti- Rabbit IgG Polyclonal Antibody . (Catalog: MBS2086047)

image 3 :

DAB staining on IHC-P; Samples:Human Cardiac Muscle Tissue; . Primary Ab: 208g/ml Rabbit Anti-Human TTN Antibody. Second Ab: 28g/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody. (Catalog: MBS2086047)

product information

catalog number :

MBS2004003

products type :

Antibody

products full name :

Polyclonal Antibody to Titin (TTN)

products short name :

[Titin (TTN)]

other names :

[titin isoform N2BA; Titin; titin; titin; Connectin; Rhabdomyosarcoma antigen MU-RMS-40.14]

products gene name :

[TTN]

other gene names :

[TTN; TTN; TMD; CMH9; CMD1G; CMPD4; EOMFC; HMERF; MYLK5; SALMY; LGMD2J]

clonality :

Polyclonal

host :

Rabbit

purity :

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

concentration :

0.64mg/ml

storage stability :

Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP)

app notes :

Western blotting: 0.5-2 ug/mL;. Immunohistochemistry: 5-20 ug/mL;. Immunocytochemistry: 5-20 ug/mL;. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochmistry (IHC)

other info1 :

Organism Species: Homo sapiens (Human). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant TTN (Pro33779~Gln34025) expressed in E.coli

ncbi gi num :

378925625

ncbi acc num :

NP_001243779.1

ncbi gb acc num :

NM_001256850.1

uniprot acc num :

Q8WZ42

ncbi pathways :

Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Hemostasis Pathway (1269340); Hypertrophic Cardiomyopathy (HCM) Pathway (114229); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Muscle Contraction Pathway (1269868); Platelet Activation, Signaling And Aggregation Pathway (1269350); Platelet Degranulation Pathway (1269367); Response To Elevated Platelet Cytosolic Ca2+ Pathway (1269365); Striated Muscle Contraction Pathway (1269869)

ncbi summary :

This gene encodes a large abundant protein of striated muscle. The product of this gene is divided into two regions, a N-terminal I-band and a C-terminal A-band. The I-band, which is the elastic part of the molecule, contains two regions of tandem immunoglobulin domains on either side of a PEVK region that is rich in proline, glutamate, valine and lysine. The A-band, which is thought to act as a protein-ruler, contains a mixture of immunoglobulin and fibronectin repeats, and possesses kinase activity. An N-terminal Z-disc region and a C-terminal M-line region bind to the Z-line and M-line of the sarcomere, respectively, so that a single titin molecule spans half the length of a sarcomere. Titin also contains binding sites for muscle associated proteins so it serves as an adhesion template for the assembly of contractile machinery in muscle cells. It has also been identified as a structural protein for chromosomes. Alternative splicing of this gene results in multiple transcript variants. Considerable variability exists in the I-band, the M-line and the Z-disc regions of titin. Variability in the I-band region contributes to the differences in elasticity of different titin isoforms and, therefore, to the differences in elasticity of different muscle types. Mutations in this gene are associated with familial hypertrophic cardiomyopathy 9, and autoantibodies to titin are produced in patients with the autoimmune disease scleroderma. [provided by RefSeq, Feb 2012]

uniprot summary :

Titin: a protein kinase that is abundant in striated muscle. Is divided into two regions, a N-terminal I-band and a C-terminal A-band. The I-band, which is the elastic part of the molecule, contains two regions of tandem immunoglobulin domains on either side of a PEVK region that is rich in proline, glutamate, valine and lysine. The A-band, which is thought to act as a protein-ruler, contains a mixture of immunoglobulin and fibronectin repeats, and possesses kinase activity. A N-terminal Z-disc region and a C-terminal M-line region bind to the Z-line and M-line of the sarcomere respectively so that a single titin molecule spans half the length of a sarcomere. Titin also contains binding sites for muscle associated proteins so it serves as an adhesion template for the assembly of contractile machinery in muscle cells. It has also been identified as a structural protein for chromosomes. Mutations are associated with familial hypertrophic cardiomyopathy 9 and autoantibodies to titin are produced in patients with the autoimmune disease scleroderma. Protein type: CAMK group; EC 2.7.11.1; Kinase, protein; MLCK family; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor). Chromosomal Location of Human Ortholog: 2q31.2. Cellular Component: condensed nuclear chromosome; cytosol; extracellular exosome; extracellular region; I band; M band; muscle myosin complex; Z disc. Molecular Function: actin filament binding; actinin binding; ATP binding; calcium ion binding; calmodulin binding; enzyme binding; identical protein binding; muscle alpha-actinin binding; protease binding; protein binding; protein kinase binding; protein self-association; protein serine/threonine kinase activity; protein tyrosine kinase activity; structural constituent of muscle; telethonin binding. Biological Process: cardiac muscle contraction; cardiac muscle fiber development; cardiac muscle hypertrophy; cardiac muscle morphogensis; cardiac myofibril assembly; detection of muscle stretch; mitotic chromosome condensation; muscle contraction; muscle filament sliding; muscle thin filament assembly; peptidyl-tyrosine phosphorylation; platelet degranulation; positive regulation of gene expression; positive regulation of protein secretion; protein kinase A signaling; regulation of catalytic activity; regulation of protein kinase activity; response to calcium ion; sarcomere organization; sarcomerogenesis; skeletal muscle myosin thick filament assembly; striated muscle contraction. Disease: Cardiomyopathy, Dilated, 1g; Hereditary Myopathy With Early Respiratory Failure; Muscular Dystrophy, Limb-girdle, Type 2j; Myopathy, Early-onset, With Fatal Cardiomyopathy; Tibial Muscular Dystrophy, Tardive

size1 :

0.01 mg

price1 :

105 USD

size2 :

0.02 mg

price2 :

125

size3 :

0.05 mg

price3 :

170

size4 :

0.1 mg

price4 :

220

size5 :

0.2 mg

price5 :

320

size6 :

1 mg

price6 :

760

more info or order :

MyBioSource product webpage