catalog number :
MBS2003893
products full name :
Polyclonal Antibody to Inter Alpha-Globulin Inhibitor H5 (ITIH5)
products short name :
Inter Alpha-Globulin Inhibitor H5 (ITIH5)
other names :
inter-alpha-trypsin inhibitor heavy chain H5 isoform 2; Inter-alpha-trypsin inhibitor heavy chain H5; inter-alpha-trypsin inhibitor heavy chain H5; ITI heavy chain H5; inter-alpha (globulin) inhibitor H5; inter-alpha-inhibitor heavy chain 5; inter-alpha-trypsin inhibitor heavy chain family, member 5
products gene name :
ITIH5
other gene names :
ITIH5; ITIH5; ITI-HC5; PP14776; KIAA1953; ITI heavy chain H5; ITI-HC5
uniprot entry name :
ITIH5_HUMAN
sequence :
The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- DL NHIERLWSYL TVKELLSSWR QSNSEQEKEQ LRQKAQDLAL NYHFLTPFTS MKLRKPGLRT NQLEDTYGMS AATGPATVVQ NLREAGKQPE PDLKKTYDPR IKISKTSVDG DPHFVVDFPL SKLTVCFNID GEPGDILRLV SDHLNSGVTV NGELIGAPAP PNGHKKQ
specificity :
The antibody is a rabbit polyclonal antibody raised against ITIH5. It has been selected for its ability to recognize ITIH5 in immunohistochemical staining andwestern blotting.
purity :
Affinity Chromatography
form :
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
storage stability :
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
tested application :
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
app notes :
Western blotting: 1:50-400. Immunocytochemistry in formalin fixed cells: 1:50-500. Immunohistochemistry in formalin fixed frozen section: 1:50-500. Immunohistochemistry in paraffin section: 1:10-100. Enzyme-linked Immunosorbent Assay: 1:100-200. Optimal working dilutions must be determined by end user.
other info1 :
Immunogen: ITIH5, Mouse. Immunogen: Recombinant ITIH5 (Asp569~Gln737) with N-terminal His-Tag
expressed in E.coli. Accession No.: RPH775Mu01
other info2 :
Quality Control: Content: The quality control contains recombinant Thyroglobulin (Ser2083~Ala2333) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
ncbi acc num :
NP_116206.4
ncbi gb acc num :
NM_032817.5
ncbi mol weight :
104,576 Da
ncbi summary :
This gene encodes a heavy chain component of one of the inter-alpha-trypsin inhibitor (ITI) family members. ITI proteins are involved in extracellular matrix stabilization and in the prevention of tumor metastasis. They are also structurally related plasma serine protease inhibitors and are composed of a light chain and varying numbers of heavy chains. This family member is thought to function as a tumor suppressor in breast and thyroid cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]
uniprot summary :
ITIH5: May act as a tumor suppressor. Belongs to the ITIH family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 10p14. Cellular Component: extracellular region. Molecular Function: serine-type endopeptidase inhibitor activity. Biological Process: hyaluronan metabolic process