GSTT1 antibody | MyBioSource MBS200376 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

GSTT1 antibody

catalog :

MBS200376

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[AT38D11]

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

The recombinant human GSTT1 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSTT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: GSTT1 Recombinant protein Lane 2.: HeLa cell lysate Lane 3.: 293T cell lysate Lane 4.: Jurkat cell lysate Lane 5.: HL-60 cell lysate

image 2 :

ICC/IF analysis of GSTT1 in HepG2 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSTT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

product information

catalog number :

MBS200376

products type :

Antibody

products full name :

GSTT1 antibody

products short name :

[GSTT1]

products name syn :

[Glutathione S-transferase theta 1; Glutathione S-transferase theta 1; GSTT1; GST class-theta-1; Glutathione transferase T1-1; Glutathione S-transferase theta 1 EC 2.5.1.18; Glutathione S transferase 5; Glutathione S transferase theta 1; GST class theta 1; Glutathione transferase T1 1; GST 5 5; GST CL1,]

other names :

[Glutathione S-transferase theta 1; Glutathione S-transferase theta-1; glutathione S-transferase theta-1; glutathione S-transferase theta 1; GST class-theta-1; Glutathione transferase T1-1]

products gene name :

[GSTT1]

other gene names :

[GSTT1; GSTT1]

clonality :

Monoclonal

isotype :

IgG2b, k

clone :

[AT38D11]

host :

Mouse

reactivity :

Human

sequence length :

240

purity :

By protein-A affinity chromatography

form :

Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.

concentration :

1 mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info1 :

Antigen Species: Human. Immunogen: Recombinant human GSTT1 (1-240aa) purified from E. coli

other info2 :

Clone Note: Anti-human GSTT1 mAb, clone AT38D11, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GSTT1 protein.

products categories :

Proteases & Inhibitors

products description :

Glutathione S-transferase theta 1 (GSTT1) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes (alpha, mu, pi, theta, and zeta). The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries.

products references :

Webb G., et al. (1996) Genomics. 33 (1): 121-123.; Mainwaring GW., et al. (1996) Biochem J. 318: 297-303.

ncbi gi num :

13937911

ncbi acc num :

AAH07065

ncbi mol weight :

13,517 Da

ncbi pathways :

Biological Oxidations Pathway (1270189); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Drug Metabolism - Cytochrome P450 Pathway (83032); Drug Metabolism - Cytochrome P450 Pathway (427); Fluid Shear Stress And Atherosclerosis Pathway (1474302); Fluid Shear Stress And Atherosclerosis Pathway (1473618); Glutathione Conjugation Pathway (1270213); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (343)

ncbi summary :

The protein encoded by this gene, glutathione S-transferase (GST) theta 1 (GSTT1), is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1, GSTT2, and GSTT2B. GSTT1 and GSTT2/GSTT2B share 55% amino acid sequence identity and may play a role in human carcinogenesis. The GSTT1 gene is haplotype-specific and is absent from 38% of the population. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2015]

uniprot summary :

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide.

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage