Polyclonal Antibody to Coagulation Factor V (F5) | MyBioSource MBS2003654 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Coagulation Factor V (F5)

catalog :

MBS2003654

quantity :

0.01 mg

price :

105 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Figure. Western Blot; Sample: Recombinant F5, Mouse.

image 2 :

DABstainingonIHC-P.Samples:MouseTissue)

product information

catalog number :

MBS2003654

products type :

Antibody

products full name :

Polyclonal Antibody to Coagulation Factor V (F5)

products short name :

[Coagulation Factor V (F5)]

other names :

[coagulation factor V preproprotein; Coagulation factor V; coagulation factor V; coagulation factor V; Activated protein C cofactor]

products gene name :

[F5]

other gene names :

[F5; F5; Cf5; Cf-5; AI173222]

clonality :

Polyclonal

host :

Rabbit

purity :

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

form :

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

concentration :

1 mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Western Blot (WB); Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP).

app notes :

Western blotting: 0.5-2 ug/mL;1:500-2000. Immunohistochemistry: 5-20 ug/mL;1:50-200. Immunocytochemistry: 5-20 ug/mL;1:50-200. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Organism Species: Mus musculus (Mouse). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant Coagulation Factor V (Asp1863-Arg2161 (Accession # O88783))expressed in E.coli

other info2 :

Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2045883

ncbi gi num :

6679731

ncbi acc num :

NP_032002.1

ncbi gb acc num :

NM_007976.3

uniprot acc num :

O88783

ncbi pathways :

Asparagine N-linked Glycosylation Pathway (1367240); Blood Clotting Cascade Pathway (198388); COPII (Coat Protein 2) Mediated Vesicle Transport Pathway (1367177); Cargo Concentration In The ER Pathway (1367176); Common Pathway Of Fibrin Clot Formation (1368161); Complement And Coagulation Cascades Pathway (198335); Complement And Coagulation Cascades Pathway (83270); Complement And Coagulation Cascades Pathway (484); ER To Golgi Anterograde Transport Pathway (1367175); Formation Of Fibrin Clot (Clotting Cascade) Pathway (1368158)

ncbi summary :

This gene encodes a glycoprotein coagulation factor that plays a critical role in the process of blood coagulation and hemostasis. The encoded protein is activated by thrombin, to generate a heterodimer containing heavy and light chains held together by calcium ions. About half of the mice lacking the encoded protein die at an embryonic stage possible due to abnormal yolk-sac vasculature while the remaining animals succumbed to massive hemorrhage immediately after birth. A point mutation in this gene has been shown to cause disseminated intravascular thrombosis in the perinatal period, resulting in frequent deaths of newborn mice. [provided by RefSeq, Apr 2015]

uniprot summary :

factor V: Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin. Defects in F5 are the cause of factor V deficiency (FA5D); also known as Owren parahemophilia. It is an hemorrhagic diastesis. Defects in F5 are the cause of thrombophilia due to activated protein C resistance (THPH2). THPH2 is a hemostatic disorder due to defective degradation of factor Va by activated protein C. It is characterized by a poor anticoagulant response to activated protein C resulting in tendency to thrombosis. Defects in F5 are a cause of susceptibility to Budd- Chiari syndrome (BDCHS). A syndrome caused by obstruction of hepatic venous outflow involving either the hepatic veins or the terminal segment of the inferior vena cava. Obstructions are generally caused by thrombosis and lead to hepatic congestion and ischemic necrosis. Clinical manifestations observed in the majority of patients include hepatomegaly, right upper quadrant pain and abdominal ascites. Budd-Chiari syndrome is associated with a combination of disease states including primary myeloproliferative syndromes and thrombophilia due to factor V Leiden, protein C deficiency and antithrombin III deficiency. Budd-Chiari syndrome is a rare but typical complication in patients with polycythemia vera. Defects in F5 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in F5 are associated with susceptibility to pregnancy loss, recurrent, type 1 (RPRGL1). RPRGL1 is a common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Belongs to the multicopper oxidase family. Protein type: Protease; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1 H2.2 1 71.46 cM. Cellular Component: endoplasmic reticulum; extracellular region; extracellular space; Golgi apparatus; membrane. Molecular Function: copper ion binding; metal ion binding. Biological Process: blood circulation; blood coagulation; hemostasis

size1 :

0.01 mg

price1 :

105 USD

size2 :

0.02 mg

price2 :

125

size3 :

0.05 mg

price3 :

170

size4 :

0.1 mg

price4 :

210

size5 :

0.2 mg

price5 :

315

size6 :

1 mg

price6 :

745

more info or order :

MyBioSource product webpage