Polyclonal Antibody to Podocalyxin (PCX) | MyBioSource MBS2003629 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Podocalyxin (PCX)

catalog :

MBS2003629

quantity :

0.01 mg

price :

105 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Mouse Brain Tissue.

image 2 :

Western Blot: Sample: Recombinant PCX, Human.

image 3 :

DAB staining on IHC-P; Samples: Human Kidney Tissue

product information

catalog number :

MBS2003629

products type :

Antibody

products full name :

Polyclonal Antibody to Podocalyxin (PCX)

products short name :

[Podocalyxin (PCX)]

other names :

[podocalyxin isoform 1; Podocalyxin; podocalyxin; podocalyxin like; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; PC; PCLP-1]

products gene name :

[PCX]

other gene names :

[PODXL; PODXL; PC; PCLP; Gp200; PCLP-1; PCLP; PCLP1; PC; PCLP-1]

clonality :

Polyclonal

host :

Rabbit

purity :

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

form :

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300,50% glycerol

concentration :

0.43 mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

WB; IHC; ICC; IP.

app notes :

Western blotting: 0.5-2)Ug/mL;1:200-800. Immunohistochemistry: 5-20)Ug/mL;1:20-80. Immunocytochemistry: 5-20)g/mL;1:20-80. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

DAB staining on IHC-P; Samples: Human Kidney Cancer Tissue.

other info1 :

Organism Species: Homo sapiens (Human). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant PCX (Ala220~Ile431) expressed in E.coli

ncbi gi num :

66277202

ncbi acc num :

NP_001018121.1

ncbi gb acc num :

NM_001018111.2

uniprot acc num :

O00592

ncbi summary :

This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]

uniprot summary :

PODXL: Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. Belongs to the podocalyxin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 7q32.3. Cellular Component: apical plasma membrane; cytoplasm; extracellular space; filopodium; integral component of plasma membrane; intracellular membrane-bound organelle; lamellipodium; membrane raft; microtubule organizing center; microvillus membrane; nucleolus; plasma membrane; ruffle. Molecular Function: protein binding. Biological Process: cell adhesion; cell migration; leukocyte migration; negative regulation of cell adhesion; negative regulation of cell-cell adhesion; positive regulation of cell migration; positive regulation of cell-cell adhesion mediated by integrin; regulation of microvillus assembly

size1 :

0.01 mg

price1 :

105 USD

size2 :

0.02 mg

price2 :

125

size3 :

0.05 mg

price3 :

165

size4 :

0.1 mg

price4 :

210

size5 :

0.2 mg

price5 :

310

size6 :

1 mg

price6 :

725

more info or order :

MyBioSource product webpage