Polyclonal Antibody to Oncostatin M (OSM) | MyBioSource MBS2003523 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Oncostatin M (OSM)

catalog :

MBS2003523

quantity :

0.01 mg

price :

130 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2003523

products type :

Antibody

products full name :

Polyclonal Antibody to Oncostatin M (OSM)

products short name :

Oncostatin M (OSM)

other names :

oncostatin-M preproprotein; Oncostatin-M; oncostatin-M; oncostatin M

products gene name :

OSM

other gene names :

OSM; OSM; OSM

uniprot entry name :

ONCM_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse

sequence length :

252

sequence :

The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF- NRGCSN SSSQLLSQLQ NQANLTGNTE SLLEPYIRLQ NLNTPDLRAA CTQHSVAFPS EDTLRQLSKP HFLSTVYTTL DRVLYQLDAL RQKFL

specificity :

The antibody is a rabbit polyclonal antibody raised against OSM. It has been selected for its ability to recognize OSM in immunohistochemical staining and western blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:50-400. Immunocytochemistry in formalin fixed cells: 1:50-500. Immunohistochemistry in formalin fixed frozen section: 1:50-500. Immunohistochemistry in paraffin section: 1:10-100. Enzyme-linked Immunosorbent Assay: 1:100-200

other info1 :

Immunogen: Recombinant OSM (Asn25~Leu115) with N-terminal His-Tag expressed in E.coli.

other info2 :

Quality Control: Content: The quality control contains recombinant Thyroglobulin (Ser2083~Ala2333) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

ncbi gi num :

10092621

ncbi acc num :

NP_065391.1

ncbi gb acc num :

NM_020530.4

uniprot acc num :

P13725

ncbi mol weight :

28,484 Da

ncbi pathways :

Adipogenesis Pathway (198832); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Oncostatin M Signaling Pathway (711361); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979)

ncbi summary :

Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. [provided by RefSeq, Jul 2008]

uniprot summary :

OSM: Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration. Belongs to the LIF/OSM family. Protein type: Secreted; Cytokine; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 22q12.2. Cellular Component: extracellular space. Molecular Function: growth factor activity; oncostatin-M receptor binding; cytokine activity. Biological Process: negative regulation of hormone secretion; multicellular organismal development; tyrosine phosphorylation of Stat3 protein; tyrosine phosphorylation of Stat5 protein; positive regulation of peptidyl-serine phosphorylation; peripheral nervous system development; negative regulation of cell proliferation; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; behavioral response to pain; positive regulation of MAPKKK cascade; response to heat; positive regulation of acute inflammatory response; positive regulation of cell division; regulation of growth; negative regulation of meiosis; positive regulation of cell proliferation; tyrosine phosphorylation of Stat1 protein; positive regulation of transcription from RNA polymerase II promoter; immune response

size1 :

0.01 mg

price1 :

130 USD

size2 :

0.05 mg

price2 :

225

size3 :

0.1 mg

price3 :

290

more info or order :

MyBioSource product webpage