product summary
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company name :
MyBioSource
product type :
antibody
product name :
DHFR antibody
catalog :
MBS200340
quantity :
0.05 mL
price :
240 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
[AT5B2]
reactivity :
human
application :
western blot, ELISA, immunocytochemistry, flow cytometry, FACS, enzyme immunoassay
more info or order :
MyBioSource product webpage
image
image 1 :
MyBioSource MBS200340 image 1
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DHFR antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : Jurkat cell lysate Lane 3 : 293T cell lysate
product information
catalog number :
MBS200340
products type :
Antibody
products full name :
DHFR antibody
products short name :
[DHFR]
products name syn :
[Dihydrofolate reductase; EC 1.5.1.3]
other names :
[dihydrofolate reductase isoform 1; Dihydrofolate reductase; dihydrofolate reductase; dihydrofolate reductase]
products gene name :
[DHFR]
other gene names :
[DHFR; DHFR; DYR; DHFRP1]
clonality :
Monoclonal
isotype :
IgG1, k
clone :
[AT5B2]
host :
Mouse
reactivity :
Human
sequence length :
187
purity :
By protein-A affinity chromatography
form :
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
concentration :
1 mg/ml
storage stability :
Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.
tested application :
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
image1 heading :
Western Blot (WB)
other info1 :
Antigen Species: Human. Immunogen: Recombinant human DHFR (1-187aa) purified from E. coli
other info2 :
Clone Note: Anti-human DHFR mAb, clone AT5B2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human DHFR protein.
products description :
Dihydrofolate reductase (DHFR) catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and is a crucial enzyme for the synthesis of purines, pyrimidines and some amino acids. Inhibition of the activity of this enzyme leads to arrest of DNA synthesis and cell death. Gene expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.
products references :
Chen MJ., et al. (1984) J Biol Chem. 259 (6): 3933-3943. ; Cody V., et al. (2009) Biochemistry. 48 (8): 1702-1711.
ncbi gi num :
4503323
ncbi acc num :
NP_000782
ncbi gb acc num :
NM_000791.3
uniprot acc num :
P00374
ncbi mol weight :
15,672 Da
ncbi pathways :
Antifolate Resistance Pathway (1440526); Antifolate Resistance Pathway (1440443); Cell Cycle Pathway (1269741); Cell Cycle, Mitotic Pathway (1269763); E2F Mediated Regulation Of DNA Replication Pathway (1269774); E2F Transcription Factor Network Pathway (137934); Fluoropyrimidine Activity Pathway (198778); Folate Metabolism Pathway (198833); Folate Biosynthesis Pathway (83018); Folate Biosynthesis Pathway (404)
ncbi summary :
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]
uniprot summary :
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.
size1 :
0.05 mL
price1 :
240 USD
size2 :
0.1 mL
price2 :
315
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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