Polyclonal Antibody to Hepcidin (Hepc) | MyBioSource MBS2003276 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Hepcidin (Hepc)

catalog :

MBS2003276

quantity :

0.01 mg

price :

110 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Recombinant Hepc, Mouse.

image 2 :

DAB staining on fromalin fixed paraffin-embedded Liver tissue)

product information

catalog number :

MBS2003276

products type :

Antibody

products full name :

Polyclonal Antibody to Hepcidin (Hepc)

products short name :

[Hepcidin (Hepc)]

other names :

[hepcidin preproprotein; Hepcidin; hepcidin; hepcidin antimicrobial peptide]

products gene name :

[Hepc]

other gene names :

[Hamp; Hamp; Hepc; Hamp1; Hepc1; Hamp1; Hepc; Hepc1]

clonality :

polyclonal

host :

rabbit

purity :

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

concentration :

0.8mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 24 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP).

app notes :

Western blotting: 0.5-2ug/mL. Immunohistochemistry: 5-20ug/mL. Immunocytochemistry: 5-20ug/mL. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Organism Species: Mus musculus (Mouse). Source: Polyclonal antibody preparation. Traits: Liquid

other info2 :

Immunogen: Recombinant Hepc (Thr24~Thr83) expressed in E.coli

ncbi gi num :

14211542

ncbi acc num :

NP_115930.1

ncbi gb acc num :

NM_032541.2

uniprot acc num :

Q9EQ21

ncbi pathways :

Hfe Effect On Hepcidin Production Pathway (1458499); Iron Homeostasis Pathway (198312)

ncbi summary :

This gene encodes hepcidin, an antimicrobial peptide and master hormonal regulator of systemic iron metabolism. The encoded preproprotein is synthesized in the hepatocytes where it undergoes proteolytic processing to generate disulfide-linked mature peptides that are secreted into the bloodstream. Mice lacking the encoded protein develop multivisceral iron overlaod, with sparing of the spleen macrophages. Certain mutations in the human ortholog of this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis. This gene is located adjacent to a related hepcidin gene on chromosome 7. [provided by RefSeq, Aug 2016]

uniprot summary :

HAMP: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. Defects in HAMP are the cause of hemochromatosis type 2B (HFE2B); also known as juvenile hemochromatosis (JH). HFE2B is a disorder of iron metabolism with excess deposition of iron in the tissues, bronze skin pigmentation, hepatic cirrhosis, arthropathy and diabetes. The most common symptoms of hemochromatosis type 2 at presentation are hypogonadism and cardiomyopathy. Belongs to the hepcidin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7 B1 7 19.27 cM. Cellular Component: extracellular region; extracellular space; nucleus. Molecular Function: copper ion binding; hormone activity. Biological Process: cellular iron ion homeostasis; cellular response to extracellular stimulus; defense response to bacterium; defense response to fungus; iron ion homeostasis; JAK-STAT cascade; myeloid cell homeostasis; negative regulation of bone resorption; negative regulation of inflammatory response; negative regulation of transcription from RNA polymerase II promoter; positive regulation of macrophage activation; positive regulation of transcription from RNA polymerase II promoter

size1 :

0.01 mg

price1 :

110 USD

size2 :

0.02 mg

price2 :

125

size3 :

0.05 mg

price3 :

175

size4 :

0.1 mg

price4 :

225

size5 :

0.2 mg

price5 :

330

size6 :

1 mg

price6 :

785

more info or order :

MyBioSource product webpage