antibody

Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 2 (ADAMTS2)

MBS2003250

0.05 mg

345 USD

polyclonal

rabbit

nonconjugated

human, cow

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

Antibody

Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 2 (ADAMTS2)

A Disintegrin And Metalloproteinase With Thrombospondin 2 (ADAMTS2)

A disintegrin and metalloproteinase with thrombospondin motifs 2 isoform 1 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 2; A disintegrin and metalloproteinase with thrombospondin motifs 2; procollagen I N-proteinase; procollagen N-endopeptidase; procollagen I/II amino propeptide-processing enzyme; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 2; ADAM metallopeptidase with thrombospondin type 1 motif, 2; Procollagen I N-proteinase; PC I-NP; Procollagen I/II amino propeptide-processing enzyme; Procollagen N-endopeptidase

ADAMTS2

ADAMTS2; ADAMTS2; NPI; PNPI; PCINP; PCPNI; PCI-NP; PC I-NP; ADAM-TS2; ADAMTS-2; ADAMTS-3; PCINP; PCPNI; ADAM-TS 2; ADAM-TS2; ADAMTS-2; PC I-NP; pNPI

ATS2_HUMAN

Polyclonal

IgG

Rabbit

Bovine

1211

The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSGS-QLPGLHY SMNEQCRFDF GLGYMMCTAF RTFDPCKQLW CSHPDNPYFC KTKKGPPLDG TMCAPGKHCF KGHCIWLTPD ILKRDGNWGA WSPFGSCSRT CGTGVKFRTR QCDNPHPANG GRTCSGLAYD FQLCNSQDCP

The antibody is a rabbit polyclonal antibody raised against ADAMTS2. It has been selected for its ability to recognize ADAMTS2 in immunohistochemical staining andwestern blotting.

Affinity Chromatography

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

200ug/ml

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200

Immunogen: Recombinant ADAMTS2 (Gln474~Pro610) expressed in E.coli.

Quality Control: Content: The quality control contains recombinant CRP (Phe17~Pro224) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

110825974

NP_055059.2

NM_014244.4

O95450

134,755 Da

Collagen Biosynthesis And Modifying Enzymes Pathway 645289!!Collagen Formation Pathway 645288!!Extracellular Matrix Organization Pathway 576262

Function: Cleaves the propeptides of type I and II collagen prior to fibril assembly. Does not act on type III collagen. May also play a role in development that is independent of its role in collagen biosynthesis. Catalytic activity: Cleaves the N-propeptide of collagen chain alpha-1(I) at Pro- -Gln and of alpha-1(II) and alpha-2(I) at Ala- -Gln. Cofactor: Binds 1 zinc ion per subunit . By similarity. Subunit structure: May belong to a multimeric complex. Binds specifically to collagen type XIV . By similarity. Subcellular location: Secreted extracellular space extracellular matrix . By similarity. Tissue specificity: Expressed at high level in skin, bone, tendon and aorta and at low levels in thymus and brain. Domain: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix. Post-translational modification: The precursor is cleaved by a furin endopeptidase . By similarity.Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion . By similarity. Involvement in disease: Ehlers-Danlos syndrome 7C (EDS7C) [MIM:225410]: A connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. Marked by extremely fragile tissues, hyperextensible skin and easy bruising. Facial skin contains numerous folds, as in the cutis laxa syndrome.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.1. Sequence similarities: Contains 1 disintegrin domain.Contains 1 peptidase M12B domain.Contains 1 PLAC domain.Contains 4 TSP type-1 domains. Caution: Has sometimes been referred to as ADAMTS3.

0.05 mg

345 USD