Polyclonal Antibody to C Reactive Protein (CRP) | MyBioSource MBS2003208 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to C Reactive Protein (CRP)

catalog :

MBS2003208

quantity :

0.01 mg

price :

115 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2003208

products type :

Antibody

products full name :

Polyclonal Antibody to C Reactive Protein (CRP)

products short name :

C Reactive Protein (CRP)

other names :

C-reactive protein; C-reactive protein; C-reactive protein; pentraxin 1; C-reactive protein, pentraxin-related

products gene name :

CRP

other gene names :

CRP; CRP; PTX1; PTX1

uniprot entry name :

CRP_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Rat

sequence length :

224

sequence :

The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEF-H EDMSKQAFVF PGVSATAYVS LEAESKKPLE AFTVCLYAHA DVSRSFSIFS YATKTSFNEI LLFWTRGQGF SIAVGGPEIL FSASEIPEVP THICATWESA TGIVELWLDG KPRVRKSLQK GYIVGTNASI ILGQEQDSYG GGFDANQSLV GDIGDVNMWD FVLSPEQINA VYVGRVFSPN VLNWRALKYE THGDVFIKPQ LWPLTDCCES

specificity :

The antibody is a rabbit polyclonal antibody raised against CRP. It has been selected for its ability to recognize CRP in immunohistochemical staining andwestern blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:50-400. Immunocytochemistry in formalin fixed cells: 1:50-500. Immunohistochemistry in formalin fixed frozen section: 1:50-500. Immunohistochemistry in paraffin section: 1:10-100. Enzyme-linked Immunosorbent Assay: 1:100-200. Optimal working dilutions must be determined by end user.

other info1 :

Immunogen: Recombinant CRP (His20~Ser230) expressed in E.coli. Immunogen Information: Recombinant CRP (His20~Ser230) with two N-terminal Tags, His-tag and T7-tag expressed in E.coli. Accession No.: RPA821Ra01

other info2 :

Quality Control: Content: The quality control contains recombinant CRP (Phe17~Pro224) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

ncbi gi num :

55770842

ncbi acc num :

NP_000558.2

ncbi gb acc num :

NM_000567.2

uniprot acc num :

P02741

ncbi mol weight :

25,039 Da

ncbi pathways :

Classical Antibody-mediated Complement Activation Pathway (106409); Complement Cascade Pathway (106405); Creation Of C4 And C2 Activators Pathway (106407); IL6-mediated Signaling Events Pathway (137932); Immune System Pathway (106386); Initial Triggering Of Complement Pathway (106406); Innate Immune System Pathway (106387); Selenium Pathway (198825)

ncbi summary :

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq, Sep 2009]

uniprot summary :

CRP: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Belongs to the pentaxin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 1q23.2. Cellular Component: extracellular space; growth cone; extracellular region; filopodium. Molecular Function: choline binding; protein binding; protein homodimerization activity; low-density lipoprotein receptor binding; complement component C1q binding; low-density lipoprotein binding; virion binding; cholesterol binding; calcium ion binding. Biological Process: protein polymerization; defense response to Gram-positive bacterium; response to ethanol; wound healing; negative regulation of vasodilation; positive regulation of superoxide release; response to lead ion; response to hypoxia; acute-phase response; opsonization; inflammatory response; complement activation, classical pathway; aging

size1 :

0.01 mg

price1 :

115 USD

size2 :

0.05 mg

price2 :

195

size3 :

0.1 mg

price3 :

250

more info or order :

MyBioSource product webpage