CD3E antibody | MyBioSource MBS200286 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CD3E antibody

catalog :

MBS200286

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[AT3H3]

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Fig. 1: The extract of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Fig. 2: The Human Recombinant protein CD3E (200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

image 2 :

ICC/IF analysis of CD3E in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CD3E antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

image 3 :

Flow cytometry analysis of CD3E in Jurkat cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

product information

catalog number :

MBS200286

products type :

Antibody

products full name :

CD3E antibody

products short name :

[CD3E]

products name syn :

[T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE]

other names :

[T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; CD3e molecule; T-cell surface antigen T3/Leu-4 epsilon chain; CD_antigen: CD3e]

products gene name :

[CD3E]

other gene names :

[CD3E; CD3E; T3E; TCRE; IMD18; T3E]

clonality :

Monoclonal

isotype :

IgG1,k

clone :

[AT3H3]

host :

Mouse

reactivity :

Human

sequence length :

207

purity :

By protein-A affinity chromatography

form :

Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.

concentration :

1mg/ml

storage stability :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

app notes :

Recommended starting dilution is 1:1000.

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

image3 heading :

Flow cytometry (FC)

other info1 :

Antigen Species: Human. Immunogen: Recombinant human CD3E (23-126aa) purified from E. coli

other info2 :

Clone Note: Anti-human CD3E mAb, clone AT3H3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CD3E protein.

products categories :

Immunology

products description :

The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multi subunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.

products references :

Gold DP., et al. (1986) Nature 321 (6068): 431-434; Soudais C., et al. (1993) Nat Genet 3 (1):77-81.

ncbi gi num :

4502671

ncbi acc num :

NP_000724

ncbi gb acc num :

NM_000733.3

uniprot acc num :

P07766

ncbi pathways :

Adaptive Immune System Pathway (1269171); CXCR4-mediated Signaling Events Pathway (137910); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Costimulation By The CD28 Family Pathway (1269177); Downstream TCR Signaling Pathway (1269176); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Generation Of Second Messenger Molecules Pathway (1269175); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889)

ncbi summary :

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

uniprot summary :

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell developement. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage