Polyclonal Antibody to A Disintegrin And Metalloprotease 10 (ADAM10) | MyBioSource MBS2002720 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to A Disintegrin And Metalloprotease 10 (ADAM10)

catalog :

MBS2002720

quantity :

0.05 mg

price :

275 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2002720

products type :

Antibody

products full name :

Polyclonal Antibody to A Disintegrin And Metalloprotease 10 (ADAM10)

products short name :

A Disintegrin And Metalloprotease 10 (ADAM10)

other names :

A disintegrin and metalloproteinase with thrombospondin motifs 10 isoform 1 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 10; A disintegrin and metalloproteinase with thrombospondin motifs 10; ADAM-TS 10; zinc metalloendopeptidase; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 10; ADAM metallopeptidase with thrombospondin type 1 motif, 10

products gene name :

ADAM10

other gene names :

ADAMTS10; ADAMTS10; WMS; WMS1; ADAM-TS10; ADAMTS-10; ADAM-TS 10; ADAM-TS10; ADAMTS-10

uniprot entry name :

ATS10_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

1103

sequence :

The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF- R ATSGDKLNNN KFSLCSIRNI SQVLEKKRNN CFVESGQPIC GNGMVEQGEE CDCGYSDQCKDECCFDANQP EGRKCKLKPG KQCSPSQGPC CTAQCAFKSK SEKCRDDSDC AREGICNGFT ALCPASDPKP NFTDCNRHTQ VCINGQCAGS ICEKYGLEEC TCASSDGKDD KELCHVCCMK KMDPSTCAST GSVQWSRHFS GRTITLQPGS PCNDFRGYCD V

specificity :

The antibody is a rabbit polyclonal antibody raised against ADAM10. It has been selected for its ability to recognize ADAM10 in immunohistochemical staining andwestern blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200

other info1 :

Immunogen: Recombinant ADAM10 (Arg420~Val641) expressed in E.coli.

other info2 :

Quality Control: Content: The quality control contains recombinant Thyroglobulin (Ser2083~Ala2333) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

ncbi gi num :

537544566

ncbi acc num :

NP_112219.3

ncbi gb acc num :

NM_030957.3

uniprot acc num :

Q9H324

ncbi mol weight :

120,874 Da

uniprot summary :

Function: Metalloprotease that participate in microfibrils assembly. Microfibrils are extracellular matrix components occurring independently or along with elastin in the formation of elastic tissues. Ref.4. Cofactor: Binds 1 zinc ion per subunit . By similarity. Subunit structure: Interacts with FBN1; this interaction promotes microfibrils assembly. Ref.4. Subcellular location: Secreted extracellular space extracellular matrix Ref.4. Tissue specificity: Widely expressed in adult tissues. Ref.2. Domain: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix . By similarity. Post-translational modification: Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion . By similarity. Involvement in disease: Weill-Marchesani syndrome 1 (WMS1) [MIM:277600]: A rare connective tissue disorder characterized by short stature, brachydactyly, joint stiffness, and eye abnormalities including microspherophakia, ectopia lentis, severe myopia and glaucoma.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.3 Ref.5. Sequence similarities: Contains 1 disintegrin domain.Contains 1 peptidase M12B domain.Contains 1 PLAC domain.Contains 5 TSP type-1 domains.

size :

0.05 mg

price :

275 USD

more info or order :

MyBioSource product webpage