Biotin-Linked Antibody to Keratin 18 (KRT18) | MyBioSource MBS2002695 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Biotin-Linked Antibody to Keratin 18 (KRT18)

catalog :

MBS2002695

quantity :

0.1 mg

price :

235 USD

clonality :

polyclonal

host :

rabbit

conjugate :

biotin

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Figure. Western Blot; Sample: Recombinant KRT18, Human.

image 2 :

Knockout Validation: Lane 1: Wild-type Hela cell lysate;;Lane 2: KRT18 knockout Hela cell lysate;;Predicted MW: 48kDa ;Observed MW: 48kDa;Primary Ab: 2ug/ml Rabbit Anti-Human KRT18 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047)

product information

catalog number :

MBS2002695

products type :

Antibody

products full name :

Biotin-Linked Antibody to Keratin 18 (KRT18)

products short name :

[Keratin 18 (KRT18)]

other names :

[keratin, type I cytoskeletal 18; Keratin, type I cytoskeletal 18; keratin, type I cytoskeletal 18; CK-18; keratin-18; cytokeratin 18; cytokeratin-18; cell proliferation-inducing protein 46; cell proliferation-inducing gene 46 protein; keratin 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18]

products gene name :

[KRT18]

other gene names :

[KRT18; KRT18; K18; CYK18; CYK18; CK-18; K18]

uniprot entry name :

K1C18_HUMAN

clonality :

Polyclonal

host :

Rabbit

purity :

Antigen-specific Affinity Chromatography.

form :

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

concentration :

0.45 mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

WB; IHC; ICC; IP.

app notes :

Western blotting: 0.5-2 ug/mL. Immunohistochemistry: 5-20 ug/mL. Immunocytochemistry: 5-20 ug/mL;. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot(WB)

image2 heading :

Knockout Validation

other info1 :

Organism Species: Homo sapiens (Human). Source: Antibody labeling. Label: Biotin. Traits: Liquid. Immunogen: Recombinant KRT18 (Asp238~Leu396) expressed in E.coli.

other info2 :

Unconjugated Antibody: The unconjugated antibody version of this item is also available as catalog #MBS2003165

products categories :

Biotin Antibody

ncbi gi num :

40354195

ncbi acc num :

NP_954657.1

ncbi gb acc num :

NM_199187.1

uniprot acc num :

P05783

ncbi pathways :

Caspase Cascade In Apoptosis Pathway (137974); EGFR1 Signaling Pathway (198782); Pathogenic Escherichia Coli Infection Pathway (83103); Pathogenic Escherichia Coli Infection Pathway (672459); Pathogenic Escherichia Coli Infection Pathway (516)

ncbi summary :

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

K18: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Defects are a cause of cryptogenic cirrhosis. Protein type: Cytoskeletal; Nucleolus. Chromosomal Location of Human Ortholog: 12q13. Cellular Component: perinuclear region of cytoplasm; keratin filament; cytoplasm; nucleolus; intermediate filament; microtubule organizing center. Molecular Function: protein binding; structural molecule activity. Biological Process: anatomical structure morphogenesis; viral reproduction; tumor necrosis factor-mediated signaling pathway; intermediate filament cytoskeleton organization and biogenesis; cell cycle; negative regulation of apoptosis; Golgi to plasma membrane CFTR protein transport. Disease: Cirrhosis, Familial

size1 :

0.1 mg

price1 :

235 USD

size2 :

0.2 mg

price2 :

350

size3 :

1 mg

price3 :

835

more info or order :

MyBioSource product webpage