Biotin-Linked Antibody to Glutathione S Transferase Alpha 1 (GSTa1) | MyBioSource MBS2002640 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Biotin-Linked Antibody to Glutathione S Transferase Alpha 1 (GSTa1)

catalog :

MBS2002640

quantity :

0.1 mg

price :

355 USD

clonality :

polyclonal

host :

rabbit

conjugate :

biotin

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2002640

products type :

Antibody

products full name :

Biotin-Linked Antibody to Glutathione S Transferase Alpha 1 (GSTa1)

products short name :

Glutathione S Transferase Alpha 1 (GSTa1)

other names :

Glutathione S-transferase alpha 1; Glutathione S-transferase A1; glutathione S-transferase A1; GST-epsilon; GST HA subunit 1; GST, class alpha, 1; GST class-alpha member 1; glutathione S-transferase 2; glutathione S-aryltransferase A1; glutathione S-alkyltransferase A1; S-(hydroxyalkyl)glutathione lyase A1; glutathione S-transferase Ha subunit 1; glutathione S-transferase alpha 1; GST HA subunit 1; GST class-alpha member 1; GST-epsilon; GSTA1-1; GTH1

products gene name :

GSTa1

other gene names :

GSTA1; GSTA1; GST2; GTH1; GSTA1-1

uniprot entry name :

GSTA1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

222

sequence :

The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGS-AEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LlDMYIEGIA DLGEMILLLP VCPPEEKDAK LALlKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLlSSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF

specificity :

The antibody is a rabbit polyclonal antibody raised against GSTa1 conjugated to biotin. It has been selected for its ability to recognize GSTa1 in immunohistochemical staining andwestern blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunohistochemistry (IHC), (IHC-F), (IHC-P), ELISA (EIA), Western Blot (WB), (ICC)

app notes :

Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200

other info1 :

Immunogen: Recombinant GSTa1 (Ala2-Phe222) expressed in E.coli.

other info2 :

Conjugation: Biotin. QUALITY CONTROL: Content: The quality control contains recombinant GSTa1 (Ala2-Phe222) disposed in loading buffer. Usage: 10ul per well when 3,3'-Oiaminobenzidine(OAB) as the substrate. 5ul per well when used in enhanced chemilumescent (ECl). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pHS.S), 2% SOS, 200mM NaCI, 50% glycerol, BPB 0.01 %, NaN3 0.02%.

products categories :

Biotin Antibody

ncbi gi num :

31657185

ncbi acc num :

AAH53578.1

uniprot acc num :

P08263

ncbi mol weight :

25,631 Da

ncbi pathways :

4-hydroxy-2-nonenal Detoxification Pathway (703096); 4-hydroxy-2-nonenal Detoxification Pathway (703144); AhR Pathway (755436); Biological Oxidations Pathway (105698); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Drug Metabolism - Cytochrome P450 Pathway (83032); Drug Metabolism - Cytochrome P450 Pathway (427); Glutathione Conjugation Pathway (105727); Glutathione Metabolism Pathway (82973)

ncbi summary :

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver. In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity thereby protecting the cells from reactive oxygen species and the products of peroxidation. [provided by RefSeq, Jul 2008]

uniprot summary :

GSTA1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Alpha family. Protein type: EC 2.5.1.18; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Other Amino Acids Metabolism - glutathione; Transferase; Xenobiotic Metabolism - metabolism by cytochrome P450. Chromosomal Location of Human Ortholog: 6p12.1. Cellular Component: cytosol. Molecular Function: glutathione transferase activity. Biological Process: glutathione metabolic process; epithelial cell differentiation; metabolic process; xenobiotic metabolic process

size1 :

0.1 mg

price1 :

355 USD

more info or order :

MyBioSource product webpage