catalog number :
MBS2002500
products full name :
Polyclonal Antibody to Nephronectin (NPNT)
products short name :
Nephronectin (NPNT)
other names :
nephronectin isoform B; Nephronectin; nephronectin; preosteoblast EGF-like repeat protein with MAM domain; nephronectin; Preosteoblast EGF-like repeat protein with MAM domain; Protein EGFL6-like
products gene name :
NPNT
other gene names :
NPNT; NPNT; POEM; EGFL6L; EGFL6L; POEM
uniprot entry name :
NPNT_HUMAN
specificity :
The antibody is a rabbit polyclonal antibody raised against NPNT. It has been selected for its ability to recognize NPNT in immunohistochemical staining andwestern blotting.
purity :
Antigen-specific Affinity Chromatography.
form :
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
tested application :
WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
app notes :
Western blotting: 0.5-2ug/ml. Immunocytochemistry in formalin fixed cells: 5-20ug/ml. Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml. Immunohistochemistry in paraffin section: 5-20ug/ml. Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Optimal working dilutions must be determined by end user
other info1 :
Source: Polyclonal antibody preparation. Traits: Liquid
other info2 :
Immunogen: Recombinant NPNT (Leu418~Arg565) expressed in E.coli.
ncbi acc num :
NP_001028219.1
ncbi gb acc num :
NM_001033047.2
uniprot summary :
NPNT: Functional ligand of integrin alpha-8/beta-1 in kidney development. Regulates the expression of GDNF with integrin alpha- 8/beta-1 which is essential for kidney development. May also play a role in the development and function of various tissues, regulating cell adhesion, spreading and survival through the binding of several integrins. Belongs to the nephronectin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 4q24. Cellular Component: proteinaceous extracellular matrix; smooth muscle contractile fiber; extracellular region; basement membrane. Molecular Function: integrin binding; calcium ion binding. Biological Process: positive regulation of osteoblast differentiation; extracellular matrix organization and biogenesis; establishment of protein localization; ureteric bud development; ureteric bud branching; cell-matrix adhesion; cell-cell adhesion mediated by integrin; positive regulation of transforming growth factor beta receptor signaling pathway; positive regulation of smooth muscle contraction