Polyclonal Antibody to Chymase 1, Mast Cell (CMA1) | MyBioSource MBS2002348 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Chymase 1, Mast Cell (CMA1)

catalog :

MBS2002348

quantity :

0.01 mg

price :

110 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Lane1: Mouse Large Intestine Tissue; Lane2: Mouse Testis Tissue; Lane3: Human PC-3 Cells.

image 2 :

Western Blot: Sample: Recombinant CMA1, Mouse.

image 3 :

DAB staining on IHC-P; Samples: Mouse Lung Tissue

product information

catalog number :

MBS2002348

products type :

Antibody

products full name :

Polyclonal Antibody to Chymase 1, Mast Cell (CMA1)

products short name :

[Chymase 1, Mast Cell (CMA1)]

other names :

[chymase preproprotein; Chymase; chymase; chymase 1, mast cell; Alpha-chymase; Mast cell chymase 1; Mast cell protease 5; mMCP-5; Mast cell protease I]

products gene name :

[CMA1]

other gene names :

[Cma1; Cma1; Mcp5; Mcp-5; Mcpt5; MMCP-5; Mcpt5; mMCP-5]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

purity :

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

form :

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

concentration :

0.37 mg/ml

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

WB; IHC; ICC; IP.

app notes :

Western blotting: 0.5-2 ug/mL;1:180-740. Immunohistochemistry: 5-20 ug/mL;1:18-74. Immunocytochemistry: 5-20 ug/mL;1:18-74. Optimal working dilutions must be determined by end user

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

DAB staining on IHC-P; Samples: Mouse Uterine Fibroids Tissue.

other info1 :

Organism Species: Mus musculus (Mouse). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant CMA1 (Leu9~Asn247 (Accession # P21844)) expressed in E.coli

other info2 :

Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2078261

ncbi gi num :

6857812

ncbi acc num :

NP_034910.1

ncbi gb acc num :

NM_010780.3

uniprot acc num :

P21844

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (1367347); Degradation Of The Extracellular Matrix Pathway (1367346); Extracellular Matrix Organization Pathway (1367333); Lung Fibrosis Pathway (1458483); Metabolism Of Angiotensinogen To Angiotensins Pathway (1367277); Metabolism Of Proteins Pathway (1367207); Peptide Hormone Metabolism Pathway (1367274); Renin-angiotensin System Pathway (83272); Renin-angiotensin System Pathway (486); Signal Transduction Pathway (1366876)

ncbi summary :

This gene encodes a serine protease that belongs to the peptidase family S1. It is expressed in mast cells and is thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. The encoded preproprotein undergoes proteolytic processing to generate a functional enzyme with elastase-like activity. Mice lacking the encoded protein exhibit significant attenuation of ischemia-reperfusion injury of the skeletal muscle. This gene is located in a cluster of related mast cell protease genes on chromosome 14. [provided by RefSeq, Nov 2015]

uniprot summary :

CMA1: Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. Belongs to the peptidase S1 family. Granzyme subfamily. Protein type: EC 3.4.21.39; Protease; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 14 C3 14 28.19 cM. Cellular Component: extracellular matrix; extracellular region; intracellular; secretory granule. Molecular Function: endopeptidase activity; hydrolase activity; peptidase activity; peptide binding; serine-type endopeptidase activity; serine-type peptidase activity. Biological Process: interleukin-1 beta biosynthetic process; positive regulation of angiogenesis; proteolysis

size1 :

0.01 mg

price1 :

110 USD

size2 :

0.02 mg

price2 :

130

size3 :

0.05 mg

price3 :

185

size4 :

0.1 mg

price4 :

230

size5 :

0.2 mg

price5 :

345

size6 :

1 mg

price6 :

825

more info or order :

MyBioSource product webpage