Polyclonal Antibody to Deiodinase, Iodothyronine, Type II (DIO2) | MyBioSource MBS2002305 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Deiodinase, Iodothyronine, Type II (DIO2)

catalog :

MBS2002305

quantity :

0.01 mg

price :

140 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2002305

products type :

Antibody

products full name :

Polyclonal Antibody to Deiodinase, Iodothyronine, Type II (DIO2)

products short name :

Deiodinase, Iodothyronine, Type II (DIO2)

other names :

Deiodinase, iodothyronine, type II; Type II iodothyronine deiodinase; type II iodothyronine deiodinase; type 2 DI; type-II 5'deiodinase; type-II 5'-deiodinase; thyroxine deiodinase, type II; type 2 iodothyronine deiodinase; deiodinase, iodothyronine, type II; 5DII; DIOII; Type 2 DI; Type-II 5'-deiodinase

products gene name :

DIO2

other gene names :

DIO2; DIO2; D2; 5DII; SelY; DIOII; TXDI2; ITDI2; TXDI2

uniprot entry name :

IOD2_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Rattus norvegicus (Rat)

sequence length :

273

sequence :

The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE ALKDAQTGST SGSGHHHHHH SAGLVPRGST AIGMKETAAA KFERQHMDSP DLGTLEVLFQ GPLGSEF- S VILLKHVALL LSRSKSTRGE WRRMLTSEGL RCVWNSFLLD AYKQVKLGED APNSSVVHVS NPEAGNNCAS EKTADGAECH LLDFASAERP LVVNFGSATU PPFTRQLPAF RQLVEEFSSV ADFLLVYIDE AHPSDGWAVP GDSSMSFEVK KHRNQEDRCA AAHQLLERFS LPPQCQVVAD RMDNNANVAY GVAFERVCIV QRRKIAYLGG KGPFSYNLQE VRSWLEKNF

specificity :

The antibody is a rabbit polyclonal antibody raised against DIO2. It has been selected for its ability to recognize DIO2 in immunohistochemical staining and western blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:50-400. Immunocytochemistry in formalin fixed cells: 1:50-500. Immunohistochemistry in formalin fixed frozen section: 1:50-500. Immunohistochemistry in paraffin section: 1:10-100. Enzyme-linked Immunosorbent Assay: 1:100-200. Optimal working dilutions must be determined by end user.

ncbi gi num :

223462734

ncbi acc num :

AAI36515.1

uniprot acc num :

Q92813

ncbi pathways :

Amine-derived Hormones Pathway (160983); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Regulation Of Thyroid Hormone Activity Pathway (160986); Selenium Metabolism And Selenoproteins Pathway (198846); Selenium Pathway (198825); Thyroxine Biosynthesis Pathway (160985); Thyroid Hormone Metabolism I (via Deiodination) Pathway (545360); Thyroid Hormone Metabolism I (via Deiodination) Pathway (139512); Thyroid Hormone Metabolism II (via Conjugation And/or Degradation) Pathway (545361)

ncbi summary :

The protein encoded by this gene belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It is highly expressed in the thyroid, and may contribute significantly to the relative increase in thyroidal T3 production in patients with Graves disease and thyroid adenomas. This protein contains selenocysteine (Sec) residues encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

uniprot summary :

DIO2: Responsible for the deiodination of T4 (3,5,3 ,5 - tetraiodothyronine) into T3 (3,5,3 -triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development. Belongs to the iodothyronine deiodinase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; EC 1.97.1.10; Oxidoreductase. Chromosomal Location of Human Ortholog: 14q24.2-q24.3. Cellular Component: membrane; integral to membrane; plasma membrane. Molecular Function: thyroxine 5 -deiodinase activity; selenium binding; ubiquitin protein ligase binding. Biological Process: thyroid hormone generation; hormone biosynthetic process; selenocysteine incorporation; thyroid hormone metabolic process

size1 :

0.01 mg

price1 :

140 USD

size2 :

0.05 mg

price2 :

250

size3 :

0.1 mg

price3 :

345

more info or order :

MyBioSource product webpage