Polyclonal Antibody to Granzyme A (GZMA) | MyBioSource MBS2002271 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Granzyme A (GZMA)

catalog :

MBS2002271

quantity :

0.01 mg

price :

130 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2002271

products type :

Antibody

products full name :

Polyclonal Antibody to Granzyme A (GZMA)

products short name :

Granzyme A (GZMA)

other names :

granzyme A, partial; Granzyme A; granzyme A; HF; h factor; CTL tryptase; fragmentin-1; cytotoxic T-lymphocyte proteinase 1; Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease); granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3); CTL tryptase; Cytotoxic T-lymphocyte proteinase 1; Fragmentin-1; Granzyme-1; Hanukkah factor

products gene name :

GZMA

other gene names :

GZMA; GZMA; HFSP; CTLA3; CTLA3; HFSP; H factor; HF

uniprot entry name :

GRAA_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse

sequence length :

sequence :

The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF-II GGDTVVPHSR PYMALLKLSS NTICAGALIE KNWVLTAAHC NVGKRSKFIL GAHSINKEPE QQILTVKKAF PYPCYDEYTR EGDLQLVRLK KKATVNRNVA ILHLPKKGDD VKPGTRCRVA GWGRFGNKSA PSETLREVNI TVIDRKICND EKHYNFHPVI GLNMICAGDL RGGKDSCNGD SGSPLLCDGI LRGITSFGGE KCGDRRWPGV YTFLSDKHLN WIKKIMKGSV

specificity :

The antibody is a rabbit polyclonal antibody raised against GZMA. It has been selected for its ability to recognize GZMA in immunohistochemical staining andwestern blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:50-400. Immunocytochemistry in formalin fixed cells: 1:50-500. Immunohistochemistry in formalin fixed frozen section: 1:50-500. Immunohistochemistry in paraffin section: 1:10-100. Enzyme-linked Immunosorbent Assay: 1:100-200. Optimal working dilutions must be determined by end user.

other info1 :

Immunogen: GZMA, Mouse. Immunogen Information: Recombinant GZMA (Ile29~Val260) with N-terminal His-Tag expressed in E.coli. Accession No.: RPA599Mu01

other info2 :

Quality Control: Content: The quality control contains recombinant CRP (Phe17~Pro224) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

ncbi gi num :

4096788

ncbi acc num :

AAD00009.1

uniprot acc num :

P12544

ncbi mol weight :

28,999 Da

ncbi pathways :

IL12-mediated Signaling Events Pathway (137922); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462)

ncbi summary :

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. [provided by RefSeq, Jul 2008]

uniprot summary :

GZMA: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after Lys-31 and destroys its oxidative repair activity. Involved in apoptosis. Homodimer; disulfide-linked. Interacts with APEX1. Dexamethasone (DEX) induces expression of isoform beta and represses expression of isoform alpha. The alteration in expression is mediated by binding of glucocorticoid receptor to independent promoters adjacent to the alternative first exons of isoform alpha and isoform beta. Belongs to the peptidase S1 family. Granzyme subfamily. 2 isoforms of the human protein are produced by alternative promoter. Protein type: Apoptosis; Secreted; EC 3.4.21.78; Protease; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 5q11-q12. Cellular Component: extracellular region; immunological synapse; nucleus. Molecular Function: protein binding; protein homodimerization activity; serine-type endopeptidase activity. Biological Process: proteolysis involved in cellular protein catabolic process; apoptosis; positive regulation of apoptosis; cytolysis; negative regulation of oxidoreductase activity; negative regulation of endodeoxyribonuclease activity; immune response; negative regulation of DNA binding

size1 :

0.01 mg

price1 :

130 USD

size2 :

0.05 mg

price2 :

225

size3 :

0.1 mg

price3 :

290

more info or order :

MyBioSource product webpage