Polyclonal Antibody to Osteocalcin (OC) | MyBioSource MBS2002197 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Osteocalcin (OC)

catalog :

MBS2002197

quantity :

0.01 mg

price :

135 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2002197

products type :

Antibody

products full name :

Polyclonal Antibody to Osteocalcin (OC)

products short name :

Osteocalcin (OC)

other names :

osteocalcin preproprotein; Osteocalcin; osteocalcin; bone Gla protein; gamma-carboxyglutamic acid-containing protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate (gla) protein; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein

products gene name :

other gene names :

BGLAP; BGLAP; OC; BGP; OCN; BGP

uniprot entry name :

OSTCN_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Rat

sequence length :

100

sequence :

The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- ALTAFCLSDL AGAKPSDSES DKAFMSKQEG SKVVNRLRRY LNNGLGAPAP YPDPLEPHRE VCELNPNCDE LADHIGFQDA

specificity :

The antibody is a rabbit polyclonal antibody raised against OC. It has been selected for its ability to recognize OC in immunohistochemical staining andwestern blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200

other info1 :

Immunogen: Recombinant OC (Ala11~Thr98) expressed in E.coli.

other info2 :

Quality Control: Content: The quality control contains recombinant CRP (Phe17~Pro224) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

ncbi gi num :

40316933

ncbi acc num :

NP_954642.1

ncbi gb acc num :

NM_199173.4

uniprot acc num :

P02818

ncbi mol weight :

10,963 Da

ncbi pathways :

Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); FGF Signaling Pathway (137989); Glucocorticoid Receptor Regulatory Network Pathway (138014); Interleukin-11 Signaling Pathway (698753); M Phase Pathway (105812); Mitotic Anaphase Pathway (105818); Mitotic Metaphase And Anaphase Pathway (730317); Mitotic Prometaphase Pathway (105815); Notch-mediated HES/HEY Network Pathway (169347)

ncbi summary :

This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]

uniprot summary :

osteocalcin: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Belongs to the osteocalcin/matrix Gla protein family. Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q22. Cellular Component: Golgi apparatus; extracellular space; rough endoplasmic reticulum; cytoplasm; dendrite; perikaryon. Molecular Function: structural constituent of bone; hydroxyapatite binding; calcium ion binding; structural molecule activity. Biological Process: response to drug; response to gravity; regulation of bone resorption; response to glucocorticoid stimulus; cell aging; response to testosterone stimulus; bone mineralization; osteoblast development; odontogenesis; osteoblast differentiation; regulation of osteoclast differentiation; response to ethanol; response to vitamin D; response to vitamin K; response to zinc ion; response to estrogen stimulus; response to mechanical stimulus; response to hydroxyisoflavone; response to activity; cell adhesion; regulation of bone mineralization; skeletal development

size1 :

0.01 mg

price1 :

135 USD

size2 :

0.05 mg

price2 :

230

size3 :

0.1 mg

price3 :

310

more info or order :

MyBioSource product webpage