product summary
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company name :
MyBioSource
product type :
antibody
product name :
Polyclonal Antibody to N-Methylpurine DNA Glycosylase (MPG)
catalog :
MBS2002169
quantity :
0.01 mg
price :
135 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS2002169
products type :
Antibody
products full name :
Polyclonal Antibody to N-Methylpurine DNA Glycosylase (MPG)
products short name :
N-Methylpurine DNA Glycosylase (MPG)
other names :
N-methylpurine-DNA glycosylase; DNA-3-methyladenine glycosylase; DNA-3-methyladenine glycosylase; 3-alkyladenine DNA glycosylase; 3-methyladenine DNA glycosidase; proliferation-inducing protein 11; proliferation-inducing protein 16; N-methylpurine-DNA glycosylase, MPG; CRA36.1 (3-methyl-adenine DNA glycosylase); 3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on 16p; N-methylpurine-DNA glycosylase; 3-alkyladenine DNA glycosylase; 3-methyladenine DNA glycosidase; ADPG; N-methylpurine-DNA glycosylase
products gene name :
MPG
other gene names :
MPG; MPG; AAG; MDG; ADPG; APNG; Mid1; anpg; PIG11; PIG16; CRA36.1; AAG; ANPG; MID1
uniprot entry name :
3MG_HUMAN
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Mouse
sequence length :
271
sequence :
The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-LVTGY HEVGQMPAPL SRKIGQKKQR LADSEQQQTP KERLLSTPGL RRSIYFSSPE DHSGRLGPEF FDQPAVTLAR AFLGQVLVRR LADGTELRGR IVETEAYLGP EDEAAHSRGG RQTPRNRGMF MKPGTLYVYL IYGMYFCLNV SSQGAGACVL LRALEPLEGL ETMRQLRNSL RKSTVGRSLK DRELCSGPSK LCQALAIDKS FDQRDLAQDD AVWLEHGPLE SSSPAVVVAA ARIGIGHAGE WTQKPLRFYV QGSPWVSVVD RVAEQ
specificity :
The antibody is a rabbit polyclonal antibody raised against MPG. It has been selected for its ability to recognize MPG in immunohistochemical staining andwestern blotting.
purity :
Affinity Chromatography
form :
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
concentration :
200ug/ml
storage stability :
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
tested application :
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
app notes :
Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200
other info1 :
Immunogen: Recombinant MPG (Leu46~Gln315) expressed in E.coli.
other info2 :
Quality Control: Content: The quality control contains recombinant CRP (Phe17~Pro224) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
ncbi gi num :
233968
ncbi acc num :
AAB19537.1
uniprot acc num :
P29372
ncbi mol weight :
32,869 Da
ncbi pathways :
Base Excision Repair Pathway (105838); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Base-Excision Repair, AP Site Formation Pathway (105839); Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway (105848); Cleavage Of The Damaged Purine Pathway (105842); DNA Repair Pathway (105837); Depurination Pathway (105840); Displacement Of DNA Glycosylase By APE1 Pathway (105849); Recognition And Association Of DNA Glycosylase With Site Containing An Affected Purine Pathway (105841)
uniprot summary :
MPG: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions. Belongs to the DNA glycosylase MPG family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Hydrolase; Nuclear receptor co-regulator; EC 3.2.2.21. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: nucleoplasm. Molecular Function: protein binding; alkylbase DNA N-glycosylase activity; DNA-3-methyladenine glycosylase I activity; damaged DNA binding. Biological Process: DNA dealkylation; base-excision repair, AP site formation; depurination; base-excision repair; DNA repair
size1 :
0.01 mg
price1 :
135 USD
size2 :
0.05 mg
price2 :
245
size3 :
0.1 mg
price3 :
325
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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