Polyclonal Antibody to Apolipoprotein A4 (APOA4) | MyBioSource MBS2002143 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Apolipoprotein A4 (APOA4)

catalog :

MBS2002143

quantity :

0.01 mg

price :

135 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2002143

products type :

Antibody

products full name :

Polyclonal Antibody to Apolipoprotein A4 (APOA4)

products short name :

Apolipoprotein A4 (APOA4)

other names :

apolipoprotein A-IV; Apolipoprotein A-IV; apolipoprotein A-IV; apo-AIV; apoA-IV; apolipoprotein A4; apolipoprotein A-IV; Apolipoprotein A4

products gene name :

APOA4

other gene names :

APOA4; APOA4; Apo-AIV; ApoA-IV

uniprot entry name :

APOA4_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse

sequence length :

396

sequence :

The target protein is fused with two N-termina/ Tags, His-tag and S-tag and its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- QV EEFRRTVEPM GEMFNKALVQ QLEQFRQQLG PNSGEVESHL SFLEKSLREK VNSFMSTLEK KGSPDQPQA

specificity :

The antibody is a rabbit polyclonal antibody raised against APOA4. It has been selected for its ability to recognize APOA4 in immunohistochemical staining andwestern blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:50-400. Immunocytochemistry in formalin fixed cells: 1:50-500. Immunohistochemistry in formalin fixed frozen section: 1:50-500. Immunohistochemistry in paraffin section: 1:10-100. Enzyme-linked Immunosorbent Assay: 1:100-200. Optimal working dilutions must be determined by end user.

other info1 :

Immunogen: APOA4, Mouse. Immunogen Information: Recombinant APOA4 (Gln299~Ala369) with two N-terminal Tags, His-tag and S-tag expressed in E.coli. Accession No.: RPB967Mu01. UOM: 100ug

other info2 :

Quality Control: Content: The quality control contains recombinant APOA4 (Gln299~Ala369) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

ncbi gi num :

71773110

ncbi acc num :

NP_000473.2

ncbi gb acc num :

NM_000482.3

uniprot acc num :

P06727

ncbi pathways :

Amyloids Pathway (366238); Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976)

ncbi summary :

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008]

uniprot summary :

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family. Protein type: Lipid-binding; Cell adhesion; Secreted; Endoplasmic reticulum; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11q23. Cellular Component: extracellular space; chylomicron; cell surface; endoplasmic reticulum lumen; early endosome; extracellular region; cytosol. Molecular Function: lipid transporter activity; antioxidant activity; protein binding; copper ion binding; protein homodimerization activity; cholesterol transporter activity; cholesterol binding; phosphatidylcholine binding; lipid binding. Biological Process: cholesterol metabolic process; phototransduction, visible light; positive regulation of fatty acid biosynthetic process; multicellular organismal lipid catabolic process; removal of superoxide radicals; neurite regeneration; lipid homeostasis; protein-lipid complex assembly; lipoprotein metabolic process; cholesterol efflux; lipid transport; cholesterol biosynthetic process; phosphatidylcholine metabolic process; cholesterol homeostasis; leukocyte adhesion; reverse cholesterol transport; regulation of cholesterol absorption; phospholipid efflux; innate immune response in mucosa; response to lipid hydroperoxide; hydrogen peroxide catabolic process; positive regulation of lipoprotein lipase activity; retinoid metabolic process; regulation of cholesterol transport

size1 :

0.01 mg

price1 :

135 USD

size2 :

0.05 mg

price2 :

230

size3 :

0.1 mg

price3 :

310

more info or order :

MyBioSource product webpage