antibody

AKR1C1 antibody (clone AT6D10)

MBS200203

0.05 mL

240 USD

monoclonal

mouse

nonconjugated

AT6D10

western blot, ELISA, enzyme immunoassay

Antibody

AKR1C1 antibody (clone AT6D10)

AKR1C1

20-alpha-HSD; DD1/DD2; HBAB; DDH; DDH1; Aldo-keto reductase family 1; member C1 20 alpha (3 alpha) hydroxysteroid dehydrogenase; 20 alpha hydroxysteroid dehydrogenase; 2ALPHAHSD; AK1C1; MBAB; Aldo keto reductase family 1 member C1; C9; Chlordecone reductase homolog; Chlordecone reductase homolog HAKRC; DD1; Dihydrodiol dehydrogenase 1; Dihydrodiol dehydrogenase 1/2; H37; HAKRC; Hepatic dihydrodiol dehydrogenase; High affinity hepatic bile acid-binding protein; Trans-1; 2 dihydrobenzene 1; 2 diol dehydrogenase; Type II 3 alpha hydroxysteroid dehydrogenase

aldo-keto reductase family 1 member C1; Aldo-keto reductase family 1 member C1; aldo-keto reductase family 1 member C1; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-hydroxysteroid dehydrogenase; aldo-keto reductase C; chlordecone reductase homolog HAKRC; dihydrodiol dehydrogenase 1; dihydrodiol dehydrogenase 1/2; dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase; dihydrodiol dehydrogenase isoform DD1; hepatic dihydrodiol dehydrogenase; high-affinity hepatic bile acid-binding protein; indanol dehydrogenase; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; type II 3-alpha-hydroxysteroid dehydrogenase; aldo-keto reductase family 1, member C1; 20-alpha-hydroxysteroid dehydrogenase (EC:1.1.1.149); 20-alpha-HSD; Chlordecone reductase homolog HAKRC; Dihydrodiol dehydrogenase 1/2; DD1/DD2; High-affinity hepatic bile acid-binding protein; HBAB; Indanol dehydrogenase (EC:1.1.1.112); Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase (EC:1.3.1.20)

AKR1C1

AKR1C1; AKR1C1; C9; DD1; DDH; DDH1; H-37; HBAB; MBAB; HAKRC; DD1/DD2; 2-ALPHA-HSD; 20-ALPHA-HSD; DDH; DDH1; 20-alpha-HSD; DD1/DD2; HBAB

AK1C1_HUMAN

Monoclonal

IgG1,k

AT6D10

Mouse

Human

323

By protein-G affinity chromatography

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide

1mg/ml

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

ELISA (EIA), Western Blot (WB)

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000. Recommended starting dilution is 1:1000

Antigen Species: Human. Clone: Anti-human AKR1C1 mAb, clone AT6D10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human AKR1C1 protein. Immunogen: Recombinant human AKR1C1 (1-323aa) purified from E Coli

Metabolism

The human aldo-keto reductases 1C1 and 1C3 (AKR1C1 and AKR1C3) have major roles in pre receptor regulation of progesterone action. They can both convert progesterone to the less potent efficiencies. AKR1C1 and AKR1C3 also act as 3-ketosteroid reductase, and as such they can convert the most potent androgen 5alpha-DHT into 3beta-andorstandiol, which is an estrogen receptor beta ligand, and into the inactive androgen 3alpha-androstnionl, respectively.

L.C. Giudice, et al. (2004) Lancet. 3644: 1789-1799 P.G. Hompes, et al. (2007) Gynecol. Endocrinol. 23: 5-12. K.J. Berkley, et al. (2005) Science 308: 1587-1589.

5453543

NP_001344

NM_001353.5

36,788 Da

Benzo(a)pyrene Metabolism Pathway (198911); Bile Acid And Bile Salt Metabolism Pathway (106144); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Xenobiotics By Cytochrome P450 Pathway (83031); Metabolism Of Xenobiotics By Cytochrome P450 Pathway (425); Retinoid Metabolism And Transport Pathway (187208); Signal Transduction Pathway (477114)

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008]

AKR1C1: Converts progesterone to its inactive form, 20-alpha- dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. Has a low bile-binding ability. May play a role in myelin formation. Belongs to the aldo/keto reductase family. Protein type: EC 1.3.1.20; EC 1.1.1.149; EC 1.1.1.112; Xenobiotic Metabolism - metabolism by cytochrome P450; Oxidoreductase. Chromosomal Location of Human Ortholog: 10p15-p14. Cellular Component: cytosol. Molecular Function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; ketosteroid monooxygenase activity; 3-alpha-hydroxysteroid dehydrogenase (B-specific) activity; carboxylic acid binding; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; bile acid binding; aldo-keto reductase activity; 20-alpha-hydroxysteroid dehydrogenase activity; indanol dehydrogenase activity; aldehyde reductase activity; phenanthrene 9,10-monooxygenase activity. Biological Process: bile acid and bile salt transport; phototransduction, visible light; response to organophosphorus; cholesterol homeostasis; retinal metabolic process; epithelial cell differentiation; bile acid metabolic process; xenobiotic metabolic process; digestion; cholesterol absorption; progesterone metabolic process; retinoid metabolic process; protein homooligomerization

0.05 mL

240 USD

0.1 mL

315