catalog number :
MBS2001996
products full name :
Polyclonal Antibody to Lysophosphatidylcholine Acyltransferase 3 (LPCAT3)
products short name :
Lysophosphatidylcholine Acyltransferase 3 (LPCAT3)
other names :
Lysophosphatidylcholine acyltransferase 3; Lysophospholipid acyltransferase 5; lysophospholipid acyltransferase 5; lyso-PS acyltransferase; lyso-PC acyltransferase 3; putative protein similar to nessy; lysophosphatidylserine acyltransferase; 1-acylglycerophosphoserine O-acyltransferase; 1-acylglycerophosphocholine O-acyltransferase; O-acyltransferase domain-containing protein 5; membrane bound O-acyltransferase domain containing 5; O-acyltransferase (membrane bound) domain containing 5; membrane-bound O-acyltransferase domain-containing protein 5; lysophosphatidylcholine acyltransferase 3; 1-acylglycerophosphocholine O-acyltransferase (EC:2.3.1.23); 1-acylglycerophosphoserine O-acyltransferase (EC:2.3.1.n6); Lysophosphatidylcholine acyltransferase; LPCAT; Lyso-PC acyltransferase; Lysophosphatidylcholine acyltransferase 3; Lyso-PC acyltransferase 3; Lysophosphatidylserine acyltransferase; LPSAT; Lyso-PS acyltransferase; Membrane-bound O-acyltransferase domain-containing protein 5; O-acyltransferase domain-containing protein 5
products gene name :
LPCAT3
other gene names :
LPCAT3; LPCAT3; C3F; LPCAT; LPSAT; OACT5; nessy; MBOAT5; LPLAT 5; MBOAT5; OACT5; LPLAT 5; LPCAT; Lyso-PC acyltransferase; Lyso-PC acyltransferase 3; LPSAT; Lyso-PS acyltransferase; O-acyltransferase domain-containing protein 5
uniprot entry name :
MBOA5_HUMAN
sequence :
The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS- MAYLLAGYY YTATGNYDIK WTMPHCVLTL KLIGLAVDYF DGGKDQNSLS SEQQKYAIRG VPSLLEVAGF SYFYGAFLVG PQFSMNHYMK LVQGELIDIP GKIPNSIIPA LKR
specificity :
The antibody is a rabbit polyclonal antibody raised against LPCAT3. It has been selected for its ability to recognize LPCAT3 in immunohistochemical staining and western blotting.
purity :
Affinity Chromatography
form :
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
storage stability :
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
tested application :
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
app notes :
Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-200
ncbi acc num :
AAH65194.1
ncbi mol weight :
56,035 Da
ncbi pathways :
Acyl Chain Remodelling Of PC Pathway (645321); Acyl Chain Remodelling Of PE Pathway (645317); Acyl Chain Remodelling Of PS Pathway (645324); CDP-diacylglycerol Biosynthesis I Pathway (142255); Glycerophospholipid Biosynthesis Pathway (645313); Glycerophospholipid Metabolism Pathway (82989); Glycerophospholipid Metabolism Pathway (364); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Phospholipid Metabolism Pathway (645312)
uniprot summary :
MBOAT5: Acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3- phosphocholine or PC) (LPCAT activity). Catalyzes also the conversion of lysophosphatidylserine (1-acyl-2-hydroxy-sn-glycero- 3-phospho-L-serine or LPS) into phosphatidylserine (1,2-diacyl-sn- glycero-3-phospho-L-serine or PS) (LPSAT activity). Has also weak lysophosphatidylethanolamine acyltransferase activity (LPEAT activity). Favors polyunsaturated fatty acyl-CoAs as acyl donors compared to saturated fatty acyl-CoAs. Seems to be the major enzyme contributing to LPCAT activity in the liver. Lysophospholipid acyltransferases (LPLATs) catalyze the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle. Belongs to the membrane-bound acyltransferase family. Protein type: EC 2.3.1.23; EC 2.3.1.n6; Transferase; Membrane protein, multi-pass; Membrane protein, integral. Chromosomal Location of Human Ortholog: 12p13. Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane. Molecular Function: 1-acylglycerophosphocholine O-acyltransferase activity. Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process