antibody

CAV1 antibody (clone AT4C1)

MBS200195

0.05 mL

240 USD

monoclonal

mouse

nonconjugated

AT4C1

human, rat

western blot, ELISA, enzyme immunoassay

Antibody

CAV1 antibody (clone AT4C1)

CAV1

VIP 21; CAV; CAV 1; CAV1; caveolae protein 22 kD; caveolin 1 alpha isoform; caveolin 1 beta isoform; Caveolin 1 caveolae protein 22kDa; Caveolin1; cell growth-inhibiting protein 32; MSTP085; OTTHUMP00000025031; VIP 21; VIP21

caveolin-1 isoform alpha; Caveolin-1; caveolin-1; cell growth-inhibiting protein 32; caveolin 1, caveolae protein, 22kDa

CAV1

CAV1; CAV1; CGL3; PPH3; BSCL3; LCCNS; VIP21; MSTP085; CAV

CAV1_HUMAN

Monoclonal

IgG2b, k

AT4C1

Mouse

Human, Rat

178

By protein-G affinity chromatography

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide

1mg/ml

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

ELISA (EIA), Western Blot (WB)

The antibody has been tested by ELISA, Western blot to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250 ~ 1000. Recommended starting dilution is 1:500 Application: ELISA, WB

Antigen Species: Human. Clone: CAV1 mAb, clone AT4C1, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PTS protein. Immunogen: Recombinant human CAV1 (1-104aa) purified from E Coli

Signal Transduction

The identification of caveolin-1 (CAV1) as the main structural component of caveolae, together with the finding that CAV1 might serve as a molecular organizer for membrane multiprotein complexes involved in cellular traffic, endo-and transcytosis, cell adhesion and signal transduction prompted a new impulse in the research on these intracellular organells. One of the properties of CAV1 was its insolubility in cold non-ionic detergents together with apical markers in epithelial cell. Also, cholesterol is essential for caveolae formation and maintenance, and caveolae structure is highly sensitive to cholesterol depletion or treatment with cholesterol binding drugs. CAV1 tightly and specifically binds free cholesterol and artificial cholesterol containing vesicles.

Van Meer, G., et al. (2008) Nat Rev Mol Cell Biol. 9: 112-124 Todeschini, R.A. et al. (2008) Biochim Biophy Acta 1780: 421-433 Lajoie, P et al. (2007) J Cell Mol Med 11: 644-653

15451856

NP_001744

NM_001753.4

17,023 Da

ALK1 Signaling Events Pathway (137968); Androgen Receptor Signaling Pathway (198806); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Basigin Interactions Pathway (106065); Canonical Wnt Signaling Pathway (138032); Cell Surface Interactions At The Vascular Wall Pathway (106062); Direct P53 Effectors Pathway (137939); Disease Pathway (530764); EGFR1 Signaling Pathway (198782)

The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.[provided by RefSeq, Mar 2010]

Caveolin-1: May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Involved in the costimulatory signal essential for T-cell receptor (TCR)- mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3- dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Homooligomer. Interacts with GLIPR2, NOSTRIN, SNAP25 and syntaxin. Interacts with rotavirus A NSP4. Interacts (via the N- terminus) with DPP4; the interaction is direct. Interacts with CTNNB1, CDH1 and JUP. Interacts with BMX and BTK. Expressed in muscle and lung, less so in liver, brain and kidney. Belongs to the caveolin family. 2 isoforms of the human protein are produced by alternative initiation. Protein type: Nuclear receptor co-regulator; Adaptor/scaffold; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 7q31.1. Cellular Component: focal adhesion; protein complex; integral to plasma membrane; endoplasmic reticulum; basolateral plasma membrane; lipid particle; caveola; cell cortex; lipid raft; cilium; acrosomal membrane; Golgi membrane; early endosome membrane; perinuclear region of cytoplasm; apical plasma membrane; plasma membrane; intracellular; cytoplasmic vesicle; endosome. Molecular Function: identical protein binding; protein binding; enzyme binding; protease activator activity; cholesterol binding; patched binding; protein complex scaffold; structural molecule activity; nitric-oxide synthase binding; protein kinase binding; receptor binding. Biological Process: mammary gland involution; viral reproduction; negative regulation of epithelial cell differentiation; negative regulation of tyrosine phosphorylation of Stat5 protein; nitric oxide homeostasis; negative regulation of BMP signaling pathway; calcium ion homeostasis; protein localization; sequestering of lipid; regulation of the force of heart contraction by chemical signal; regulation of fatty acid metabolic process; negative regulation of protein binding; inactivation of MAPK activity; regulation of smooth muscle contraction; maintenance of cellular protein localization; skeletal muscle development; cytosolic calcium ion homeostasis; negative regulation of nitric-oxide synthase activity; cellular response to starvation; membrane depolarization; cholesterol homeostasis; response to estrogen stimulus; negative regulation of endothelial cell proliferation; T cell costimulation; negative regulation of protein ubiquitination; regulation of nitric-oxide synthase activity; response to calcium ion; response to progesterone stimulus; negative regulation of JAK-STAT cascade; leukocyte migration; lactation; vesicle organization and biogenesis; negative regulation of peptidyl-serine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of pinocytosis; nitric oxide metabolic process; mammary gland development; calcium ion transport; negative regulation of cytokine and chemokine mediated signaling pathway; angiogenesis; vasculogenesis; protein homooligomerization; vasoconstriction; cholesterol transport; negative regulation of MAPKKK cascade; negative regulation of nitric oxide biosynthetic process; MAPKKK cascade; positive regulation of metalloenzyme activity; positive regulation of peptidyl-serine phosphorylation; regulation of peptidase activity; cellular calcium ion homeostasis; triacylglycerol metabolic process; regulation of blood coagulation; positive regulation of vasoconstriction; response to hypoxia; vascular endothelial growth factor receptor signaling pathway; blood coagulation. Disease: Lipodystrophy, Congenital Generalized, Type 3; Partial Lipodystrophy, Congenital Cataracts, And Neurodegeneration Syndrome; Pulmonary Hypertension, Primary, 3

0.05 mL

240 USD

0.1 mL

315