antibody

Polyclonal Antibody to Mucin 1 (MUC1)

MBS2001934

0.01 mg

100 USD

polyclonal

rabbit

nonconjugated

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Antibody

Polyclonal Antibody to Mucin 1 (MUC1)

[Mucin 1 (MUC1)]

[mucin-1 isoform 2; Mucin-1; mucin-1; mucin 1, cell surface associated; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3]

[MUC1]

[MUC1; MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC; PUM; MUC-1; CA 15-3; KL-6; PUM; PEM; EMA; MUC1-NT; MUC1-alpha; MUC1-beta]

Polyclonal

Rabbit

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

0.47 mg/ml

Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, Western Blot (WB), Immunoprecipitation (IP)

Western blotting: 0.5-2 ug/mL;1:230-940. Immunohistochemistry: 5-20 ug/mL;1:23-94. Immunocytochemistry: 5-20 ug/mL;1:23-94. Optimal working dilutions must be determined by end user.

Western Blot (WB)

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

DAB staining on IHC-P; Samples: Human Kidney Tissue)

Organism Species: Homo sapiens (Human). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant MUC1 (Ser1098~Leu1255) expressed in E.coli

67189007

NP_001018016.1

NM_001018016.2

P15941

IL-7 Signaling Pathway (198857); Metabolism Of Proteins Pathway (106230); O-linked Glycosylation Of Mucins Pathway (530747); Post-translational Protein Modification Pathway (161012); Termination Of O-glycan Biosynthesis Pathway (530748)

This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]

MUC1: a large cell surface glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. Plays a role in adhesion and cell-cell interactions, metastasis and signaling. May provide a protective layer on epithelial surfaces. Direct or indirect interaction with actin cytoskeleton. Its cytoplasmic tail (MUC1CT) is involved in several signaling pathways, including those involving Ras, beta-catenin, p120 catenin, p53 and estrogen receptor alpha. MUC1CT also forms complexes with transcription factors, and then translocates to the nucleus by an unknown mechanism, where it is believed to influence the transcription of their target genes. MUC1CT has also been proposed to localize to mitochondrial membranes under conditions of genotoxic stress, where it attenuates the apoptotic pathway in response and confers resistance to apoptosis-inducing drugs. Aberrantly glycosylated forms expressed in human epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Nine alternatively spliced isoforms have been described. Isoforms 5 and 9 are secreted. Isoform 7, expressed only in tumor cells, is a receptor and binds the secreted isoform 5. The binding induces the phosphorylation of the isoform 7, alters cellular morphology and initiates cell signaling. Can bind to GRB2 adapter protein. Protein type: Actin-binding; Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Tumor suppressor. Chromosomal Location of Human Ortholog: 1q22. Cellular Component: apical plasma membrane; extracellular exosome; extracellular space; Golgi lumen; integral component of plasma membrane; nuclear chromatin; plasma membrane; vesicle. Molecular Function: p53 binding; protein binding; transcription cofactor activity. Biological Process: DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; negative regulation of cell adhesion mediated by integrin; negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; negative regulation of transcription by competitive promoter binding; O-glycan processing; positive regulation of histone H4 acetylation; regulation of transcription from RNA polymerase II promoter in response to stress; stimulatory C-type lectin receptor signaling pathway. Disease: Medullary Cystic Kidney Disease 1

0.01 mg

100 USD

0.02 mg

110

0.05 mg

150

0.1 mg

195

0.2 mg

270

1 mg

645